Discovery of a New Mechanism of 2, 3-Butanediol Stereoisomer Formation in Bacillus cereus YUF-4
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概要
- 論文の詳細を見る
A mechanism of 2, 3-butanediol(BD)stereoisomer formation was examined with respect to the BD cycle. The enzymes acetylacetoin synthase, acetylacetoin reductase(AACR), and acetylbutanediol hydrolse(ABDH), which are part of the BD cycle, were found to be present in the cell-free extract of the bacterial strain Bacillus cereus YUF-4, Two kinds of acetylbutanediol(ABD)stereoisomers were produced ill the reduction oF acetylacetoin(AAC)by AACR, which were identified as having 3R, 4R and 3S, 4R configurations by NMR spectroscopy and an enzymic method. The tyro ADD formations were found to be catalyzed independently by two respective enzymes: the former was catalyzed by a NADPH-dependent AACR(3R, 4R-ABD forming)and the latter by a NADH-dependent AACR(3S, 4R-ABD forming). The 3R, 4R-ABD was converted into R, R-BD and the 3S, 4R-ABD into R, S-BD by intracellular ABDH These findings demonstrated the existence of a new BD isomer formation mechanism derived from the BD cycle.
- 社団法人日本生物工学会の論文
- 1998-01-25
著者
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Ui Sadaharu
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Ui Sadaharu
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
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Watanabe Kazuhide
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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HOSAKA TAKESHI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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MIMURA AKlO
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Hosaka Takeshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Watanabe Kazuhide
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Mimura Aklo
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
関連論文
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- Mechanism for the Formation of 2,3-Butanediol Stereoisomers in Bacillus polymyxa
- Mechanism for the Formation of 2,3-Butanediol Stereoisomers in Klebsiella pneumoniae
- Laboratory-Scale Production of Acetoin Isomers (D(-) and L(+)) by Bacterial Fermentation
- Stereopecific and Electrophoretic Natures of Bacterial 2,3-Butanediol Dehydrogenases
- Laboratory-Scale Production of 2,3-Butanediol Isomers (D(-), L(+), and Meso) by Bacterial Fermentations
- Production of Acetylacetoin by Bacterial Fermentation
- Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)
- Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4
- Cloning, Expression and Nucleotide Sequence of the L-2, 3-Butanediol Dehydrogenase Gene from Brevibacterium saccharolyticum C-1012
- Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
- Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli
- Preparation of a Chiral Acetoinic Compound Using Transgenic Escherichia coli Expressing the 2,3-Butanediol Dehydrogenase Gene
- Increased Production of Antioxidative Sesaminol Glucosides from Sesame Oil Cake through Fermentation by Bacillus circulans Strain YUS-2(Microbiology & Fermentation Technology)
- Acetylacetoin Synthase as a Marker Enzyme for Detecting the 2,3-Butanediol Cycle
- Crystal Structure of meso-2,3-Butanediol Dehydrogenase in a Complex with NAD^+ and Inhibitor Mercaptoethanol at 1.7 A Resolution for Understanding of Chiral Substrate Recognition Mechanisms
- Discovery of a New Mechanism of 2, 3-Butanediol Stereoisomer Formation in Bacillus cereus YUF-4
- An Effective Culture of Ri Plasmid-induced Grape Hairy Roots
- Purification and Properties of Acetylacetoin Synthase form Bacillus sp. YUF-4
- Separation and Properties of Two Acetylacetoin Reductases from Bacillus cereus YUF-4
- Purification and Properties of NADPH-linked Diacetyl Reductase (S-Acetoin Forming) from Bacillus polymyxa
- Separation and Quantitation of 2, 3- Butanediol Isomers ((-), (+), and meso) by a Combined Use of Enzyme and Gas Chromatography