Purification and Properties of Acetylacetoin Synthase form Bacillus sp. YUF-4
スポンサーリンク
概要
- 論文の詳細を見る
In Bacillus sp. YUE-4,acetylacetoin synthase was induced by acetoin, while glucose inhibited the induction. The enzyme was purified 111-fold by 6 purification steps, and a further purification followed, by HPLC using a TSK gel, Phenyl-5PW RP. The resulting enzyme gave a single band with a molecular mass of 62 kDa by SDS-PAGE and 220 kDa by gel filtration. Some enzymic characteristics were studied.
- 社団法人日本農芸化学会の論文
- 1998-04-23
著者
-
Ui Sadaharu
Division of Biotechnology Yamanashi Univ.
-
Ui Sadaharu
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
-
Ui Sadaharu
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
-
Ui Sadaharu
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
-
MIMURA Akio
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
-
HOSAKA TAKESHI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
-
Hosaka T
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
-
MIZUTANI KIMIYA
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
-
Mimura A
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
-
Mimura A
Hitachi Ltd. Ibaraki Jpn
-
Mizutani Kimiya
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
-
Hosaka Takeshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
-
Mimura A
Univ. Of Yamanashi Yamanashi Jpn
-
Mimura Akiko
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
-
Mimura Akio
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
-
Ui S
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
関連論文
- PA-42 An alternative pathway of primary metabolism of termite gut protists inferred from meta-EST analysis(Symbiosis/Cell to cell interaction,Poster presentation A)
- Identification of Endosymbiotic Methanogen and Ectosymbiotic Spirochetes of Gut Protists of the Termite Coptotermes formosanus
- Cooperative Effect of Activated Charcoal and Gellan Gum on Grape Protoplast Culture(Biological Chemistry)
- Mechanism for the Formation of 2,3-Butanediol Stereoisomers in Bacillus polymyxa
- Mechanism for the Formation of 2,3-Butanediol Stereoisomers in Klebsiella pneumoniae
- Laboratory-Scale Production of Acetoin Isomers (D(-) and L(+)) by Bacterial Fermentation
- Stereopecific and Electrophoretic Natures of Bacterial 2,3-Butanediol Dehydrogenases
- Laboratory-Scale Production of 2,3-Butanediol Isomers (D(-), L(+), and Meso) by Bacterial Fermentations
- Production of Acetylacetoin by Bacterial Fermentation
- Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)
- Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4
- Cloning, Expression and Nucleotide Sequence of the L-2, 3-Butanediol Dehydrogenase Gene from Brevibacterium saccharolyticum C-1012
- Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
- Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli
- Preparation of a Chiral Acetoinic Compound Using Transgenic Escherichia coli Expressing the 2,3-Butanediol Dehydrogenase Gene
- Increased Production of Antioxidative Sesaminol Glucosides from Sesame Oil Cake through Fermentation by Bacillus circulans Strain YUS-2(Microbiology & Fermentation Technology)
- 3K10-1 Utilization of palm oil mill fiber in situ bioactivator process by Chaetomium sp. nov. MS-017
- Acetylacetoin Synthase as a Marker Enzyme for Detecting the 2,3-Butanediol Cycle
- 314 Enzymes decomposing palm oil mill fiber in fungus Ms-017 under higher temperature
- 1027 Isolation and cultivation of fungi on palm oil mill fiber under higher temperature
- Crystal Structure of meso-2,3-Butanediol Dehydrogenase in a Complex with NAD^+ and Inhibitor Mercaptoethanol at 1.7 A Resolution for Understanding of Chiral Substrate Recognition Mechanisms
- Discovery of a New Mechanism of 2, 3-Butanediol Stereoisomer Formation in Bacillus cereus YUF-4
- An Effective Culture of Ri Plasmid-induced Grape Hairy Roots
- Purification and Properties of Acetylacetoin Synthase form Bacillus sp. YUF-4
- Separation and Properties of Two Acetylacetoin Reductases from Bacillus cereus YUF-4
- Purification and Properties of NADPH-linked Diacetyl Reductase (S-Acetoin Forming) from Bacillus polymyxa
- On-Line Monitoring of Cell Concentrations by Dielectric Measurements
- Application of Ion Doping and Excimer Laser Annealing to Fabrication of Low-Temperature Polycrystalline Si Thin-Film Transistors
- Large-Area Doping for Poly-Si Thin Film Transistors Using Bucket Ion Source with an RF Plasma Cathode
- Isolation and Characterization of a Fast-Growing, Thermophilic, Hydrogenotrophic Methanogen
- Cultivation of Thermophilic Methanogen KN-15 on H_2-CO_2 under Pressurized Conditions
- Application of Two-Step Composting Process to Rice Straw Compost
- On-Line Monitoring of Cell Concentrations during Yeast Cultivation by Dielectric Measurements
- Growth of Thermophilic Methanogen KN-15 on H_2-CO_2 under Batch and Continuous Conditions
- A Two-Stpe Composting Process for Woody Resources
- Large-Area Doping Process for Fabrication of poly-Si Thin Film Transistors Using Bucket Ion Source and XeCl Excimer Laser Annealing
- Separation and Quantitation of 2, 3- Butanediol Isomers ((-), (+), and meso) by a Combined Use of Enzyme and Gas Chromatography