Separation and Properties of Two Acetylacetoin Reductases from Bacillus cereus YUF-4
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概要
- 論文の詳細を見る
The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.
- 社団法人日本農芸化学会の論文
- 1999-01-23
著者
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Ui Sadaharu
Division of Biotechnology Yamanashi Univ.
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Ui Sadaharu
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Ui Sadaharu
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
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Ui Sadaharu
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
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MIMURA Akio
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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HOSAKA TAKESHI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Hosaka T
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Mimura A
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
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Mimura A
Hitachi Ltd. Ibaraki Jpn
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Hosaka Takeshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Mimura A
Univ. Of Yamanashi Yamanashi Jpn
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Mimura Akiko
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
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Mimura Akio
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
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Ui S
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
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