On-Line Monitoring of Cell Concentrations by Dielectric Measurements

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概要

• 論文の詳細を見る

• Dielectric measurements were made on solutions with cells to establish the feasibility of measuring on-line cell concentrations in bioreactors. Excellent linear relationships, in which correlation coefficients were more than 0.99,were obtained between values of electric capacitance (frequency range : 100 kHz to 10 MHz) and concentrations of yeast cells (immobilized in calcium alginate beads), Escherichia coli, Aspergillus niger, human leukemia (K562) cells, Madin-Darby bovine kidney (MDBK) cells on microcarriers and plant suspension cells of Sesamum indicum L. It was also found that the linearities were maintained practically under cultivating conditions in which temperature, pH, and salt and substrate concentrations were changing during cultivation. It is concluded that the dielectric measurement method is a promising method for estimating on-line cell concentrations in bioreactors.

• 社団法人日本生物工学会の論文
• 1991-10-25

著者

• ASAMI KAZUHIRO

  Department of Bioengineering, Faculty of Biotechnology, Tokyo Institute of Technology

• Asami Kazuhiro

  Department Of Bioengineering Faculty Of Biotechnology Tokyo Institute Of Technology

• Mimura A

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Mimura A

  Hitachi Ltd. Ibaraki Jpn

• Mimura Akio

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Takahara Y

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Takahara Y

  Kobe Steel Ltd. Ibaraki Jpn

• Takahara Yoshimasa

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Mimura A

  Univ. Of Yamanashi Yamanashi Jpn

• Mimura Akiko

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Hanai T

  Laboratory Of Dielectrics Institute For Chemical Research Kyoto University

• Asami Kouji

  Department Of Bioengineering Faculty Of Biotechnology Tokyo Institute Of Technology

• MISHIMA KEN

  Biotechnology Research Laboratory, Kobe Steel Ltd.,

• ASAMI KOUJI

  Laboratory of Dielectrics, Institute for Chemical Research, Kyoto University

• HANAI TETSUYA

  Laboratory of Dielectrics, Institute for Chemical Research, Kyoto University

• Mishima Ken

  Biotechnology Research Laboratory Kobe Steel Ltd.

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