Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)

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概要

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• The L-2,3-butanediol dehydrogenase produced in E. coli JM109/pLBD2-CTC was purified by 5 steps. The molecular mass of this enzyme was estimated at 110 kDa and the subunit was measured to be 30 kDa. The L-BDH had some differences from the BDHs from other sources in substrate specificity, pI value, pH stability, effects of divalent cations, and organic acids.

• 社団法人日本農芸化学会の論文
• 2001-08-23

著者

• Ui Sadaharu

  Division of Biotechnology Yamanashi Univ.

• Ohkuma Moriya

  Environmental Molecular Biology Laboratory Riken

• Ohkuma M

  Enviromental Molecular Biology Lab. Riken

• Ohkuma Moriya

  Department Of Agricultural Chemistry The University Of Tokyo

• Ui Sadaharu

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Ui Sadaharu

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• Ohkuma Moriya

  Bio-recycle Project Japan Science And Technology Agency:presto Japan Science And Technology Agency:e

• Ui Sadaharu

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• TAKUSAGAWA Yusuke

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• OTAGIRI Masato

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• OHTSUKI Takashi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• MIMURA Akio

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• OHKUMA Moriya

  Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)

• Takio Koji

  Riken

• Takio Koji

  Biomolecular Characterization Division Riken

• Ohtsuki Takashi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Kudo T

  Riken (inst. Physical And Chemical Res.) Wako Jpn

• Kudo T

  Bio-recycle Project Japan Science And Technology Agency:environmental Molecular Biology Laboratory R

• Kudo T

  Environmental Molecular Biology Laboratory Riken

• Ohkuma Moriya

  Bio-recycle Project Japan Science And Technology Agency

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Mimura A

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Mimura A

  Hitachi Ltd. Ibaraki Jpn

• Otagiri Masato

  Institute For Protein Research Osaka University

• Mimura A

  Univ. Of Yamanashi Yamanashi Jpn

• Mimura Akiko

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Kudo Toshiaki

  Environmental Molecular Biology Laboratory Riken

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• Ohtsuki Takashi

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinsyu University

• Takio K

  Biomolecular Characterization Division Riken

• Mimura Akio

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• Takusagawa Yusuke

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Ui S

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

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