Detection of Molecular Ions of Non-Covalent Complexes of Ras・GDP and Ras・GppNp by MALDI-TOFMS
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概要
- 論文の詳細を見る
A detection of non-covalent complexes of Ras·GDP and Ras·GppNp (guanosine-5′-(β,γ-imido)-triphosphate; GTP analogue) using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOFMS) is reported. The Ras proteins are bound to GDP or GppNp, non-covalently, with ionic bonds. The GDP-bound Ras is inactive, while the GTP-bound Ras is active. This paper shows the possibility of the detection of the active and inactive form of a regulatory protein, which is non-covalently bound with a nucleotide, with a small sample amount by MALDI-TOFMS analyses. It was also suggested that MALDI-TOFMS is promising for the estimation of the ratio of nucleotide bound Ras protein and free apo-Ras protein.
- 日本質量分析学会の論文
- 1996-04-20
著者
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Takio Koji
Riken
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Takeuchi Ken-ichi
Biomolecular Characterization Team Riken
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Takio Koji
Biomolecular Characterization Division Riken
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Takio Koji
Division Of Biomolecular Characterization The Institute Of Physical And Chemical Research (riken)
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AKASHI Satoko
Division of Biomolecular Characterization, The Institute of Physical and Chemical Research (RIKEN)
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SHIROUZU Mikako
Protein Research Group, RIKEN Genomic Sciences Center
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SHIROUZU Mikako
Cellular Signaling Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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YOKOYAMA Shigeyuki
Cellular Signaling Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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Takio K
Biomolecular Characterization Division Riken
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Takio Koji
Division Of Biomolecular Characterization Riken
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Akashi Satoko
Division Of Biomolecular Characterization The Institute Of Physical And Chemical Research (riken)
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Yokoyama S
School Of Science The University Of Tokyo:protein Research Group Riken Genomic Sciences Center
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Yokoyama Shigeyuki
Cellular Signaling Laboratory The Institute Of Physical And Chemical Research (riken)
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