Dosage Effect of Minor Arginyl- and Isoleucy1-tRNAs on Protein Synthesis in an Escherichia coli In Vitro Coupled Transcription/Translation System
スポンサーリンク
概要
- 論文の詳細を見る
In Escherichia coli mRNA, the arginine codons AGA/AGG and the isoleucine codon AUA are rarely used with frequencies of about 0.35% and 0.41%, respectively. Six genes with a different number of these codons were expressed in an E.coli in vitro coupled transcription/translation system, which contained either tRNA prepared from E.coli cells carrying a plasmid with argU and ileX genes encoding rare tRNAs (tRNA^<arg>_<AGA/AGG> and tRNA^<ile>_<AUA>), designated codon-plus tRNA, or normal tRNA from cells lacking the plasmid. Genes having a low number of the rare codons, such as genes encoding chloramphenicol acetyltransferase and antigp120 single-chain Fv(artificially constructed to remove rare codons), were expressed at similar levels using with both tRNA preparations. On the other hand, the use of codon-plus tRNA increased the expression levels of genes having a relatively large number of the rare codons, including genes encoding archaeal proteins, green fluorescent protein of jelly fish origin, and a single-chain Fv of mouse origin, by about 20% higher than that using normal tRNA.
- 社団法人日本生物工学会の論文
- 2001-01-25
著者
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Nakano Hideo
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
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Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University:
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Yamane T
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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JIANG XIUPING
Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nago
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Nakano Hideo
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Jiang Xiuping
Laboratory Of Molecular Biotechnology Graduate School Of Biological And Agricultural Sciences Nagoya
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Yabuki Takashi
Protein Research Group Riken Genomic Sciences Center
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KIGAWA TAKANORI
Genomic Sciences Research Center, The Institute of Physical and Chemical Research (RIKEN)
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YABUKI TAKASHI
Genomic Sciences Research Center, The Institute of Physical and Chemical Research (RIKEN)
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YOKOYAMA SHIGEYUKI
Genomic Sciences Research Center, The Institute of Physical and Chemical Research (RIKEN)
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CLARK DOUGLAS
Department of Chemical Engineering, University of California
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Kigawa Takanori
Protein Research Group Riken Genomic Sciences Center
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Clark Douglas
Department Of Chemical Engineering University Of California
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Yokoyama S
School Of Science The University Of Tokyo:protein Research Group Riken Genomic Sciences Center
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Yamane Tsuneo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Nakano Hideo
Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag
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Yokoyama Shigeyuki
Cellular Signaling Laboratory The Institute Of Physical And Chemical Research (riken)
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Nakano Hideo
Department Of Bioengineering Sciences Graduate School Of Bioagricultural Sciences Nagoya University
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