NADH Production from NAD^+ Using Malic Enzyme of Pseudomonas diminuta IFO-13182
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概要
- 論文の詳細を見る
Permeable cells and a sonicate of Pseudomonas diminuta IFO-13182 produced NADH from NAD^+ by an NAD- and NADP-linked malic enzyme [L-malate : NAD(P)^+ oxidoreductase (EC 1.1.1.39)] reaction in the presence of L-malic acid and divalent metal ions. Optimum conditions for for NADH production were examined, including amalte concentration, NAD^+ concentration, pH, and buffer type. Under suitable conditions, the conversion ratio of NADH from NAD^+ reached 100% after 3 h of incubation at 30℃ and the amount of NADH accumulated was 14 μmol ml^<-1> of reaction mixture. The NADH produced in such enzyme reaction was purified in powder form. The purity of NADH produced was 96.6% from its coenzyme activity with alcohol dehyrogenase.
- 社団法人日本生物工学会の論文
- 1989-11-25
著者
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OBAYASHI AKIRA
Central Research Laboratories, Takara Shuzo Co., Ltd.,
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Obayashi A
Central Research Laboratories Takara Shuzo Co. Ltd.
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Yokoyama S
School Of Science The University Of Tokyo:protein Research Group Riken Genomic Sciences Center
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YOKOYAMA Sadaji
Central Research Laboratories, Takara Shuzo Co., Ltd.
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SUYE SHIN-ICHIRO
(Present address)Department of Chemical Engineering, Nara National College of Technology
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Suye Shin-ichiro
Central Research Laboratories Takara Shuzo Co. Ltd.:(present Office)department Of Chemical Engineeri
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Yokoyama Shigeyuki
Cellular Signaling Laboratory The Institute Of Physical And Chemical Research (riken)
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OBAYASHI Akira
Central Research Laboratories, Takara Shuzo Co., Ltd.
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