Isolation of Plasmids from Thermophilic Clostridia and Construction of Shuttle Vectors in Escherichia coli and Cellulolytic Clostridia
スポンサーリンク
概要
- 論文の詳細を見る
Four small plasmids (1.5-7.2kb) were found in three strains of thermophilic clostridia. Two shuttle vectors, pCL1 and pCS1,used in Escherichia coli and some strains of Clostridium were constructed from plasmid pTA688L or pTA688S and the E. coli plasmid pBR328. The transformation system examined in strains of thermophilic Clostridium, which have cellulolytic activities, yielded about 1-7×10^2 transformants per microgram of pCS1 DNA. The DNA from these transformants was of the same size as that of pCS1. Thus, the host-vector system could be useful for the study of genes involved in the cellulolytic enzymes of species of Clostridium.
- 公益社団法人日本生物工学会の論文
- 1989-11-25
著者
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UCHIDA Masahiro
Department of Physics, Tokyo Institute of Technology
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Uchida M
Takara Shuzo Co. Ltd. Shiga Jpn
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KUROSE NAOTAKA
Alcoholic Beverages and Foods Research Laboratories, Takara Shuzo Co. Ltd.
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Obayashi A
Central Research Laboratories Takara Shuzo Co. Ltd.
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Uchida M
Department Of Physics Tokyo Institute Of Technology
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Kurose N
Alcoholic Beverages And Foods Research Laboratories Takara Shuzo Co. Ltd.
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KUROSE Naotaka
Central Research Laboratories, Takara Shuzo Co., Ltd.
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YAGYU Junji
Central Research Laboratories, Takara Shuzo Co., Ltd.
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UCHIDA Masahiro
Central Research Laboratories, Takara Shuzo Co., Ltd.
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Kakimoto Takahiro
Central Research Laboratories Takara Shuzo Co. Ltd.
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MIYAZAKI TAKAYUKI
Central Research Laboratories, Takara Shuzo Co., Ltd.,
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OBYASHI AKIRA
Central Research Laboratories, Takara Shuzo Co., Ltd.,
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MUROOKA YOSHIKATSU
Fermentation Technology, Faculty of Engineering, Hiroshima University
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Miyazaki T
Marukin Shokuhin Kogyo Co. Ltd. Kumamoto Jpn
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Yagyu Junji
Central Research Laboratories Takara Shuzo Co. Ltd.
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Murooka Yoshikatsu
Fermentation Technology Faculty Of Engineering Hiroshima University
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