Purification and Characterization of Sequence-Specific Restriction Endonuclease MflI
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概要
- 論文の詳細を見る
Class II restriction endonuclease MflI was purified 790-fold from the crude extract of Microbacterium flavum by chromatography on Phosphocellulose, DEAE-cellulose, and Heparin-sepharose columns. The purified preparation was free from non-specific nucleases and phosphatases. The enzyme required 7 to 20 mM Mg^<++> ions but not monovalent cations for its activity, and the maximum activity was obtained at pH 8.0-8.5 in the Tris-HCl buffer system. This enzyme recognized 5'-PuGATCPy-3' in DNA and cleaved between Pu and G in this sequence. However, MflI digested DNA from the Escherichia coli Dam^- strain, but not DNA from its Dam^+ (wild type) strain, indicating that this enzyme only restricts the unmodified sequence.
- 社団法人日本生物工学会の論文
- 1984-12-25
著者
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KITA Keiko
Department of Biotechnology, Faculty of Engineering, Tottori University
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KITA KEIKO
Central Research Laboratories, Takara Shuzo Co. Ltd.
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HIRAOKA NOBUTSUGU
Central Research Laboratories, Takara Shuzo Co. Ltd.
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NAKAJIMA Hiroshi
central Research Institute, Maruha Co., Ltd.
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Kita K
Tottori Univ. Tottori Jpn
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Kita Keiko
Department Of Biotechnology Faculty Of Engineering Tottori University
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Kita Keiko
Central Research Laboratories Takara Shuzo Co. Ltd.
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Kita Keiko
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Nakajima H
Division Of Dental Biomaterials Science Department Of Restorative And Biomaterials Sciences Meikai U
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Hiraoka N
Alcoholic Beverages Research Laboratories Takara Shuzo Co.ltd.
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Hiraoka Nobutsugu
Central Research Laboratories Takara Shuzo Co. Ltd.
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OBAYASHI AKIRA
Central Research Laboratories, Takara Shuzo Co., Ltd.,
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Obayashi A
Central Research Laboratories Takara Shuzo Co. Ltd.
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