Characterization and Overproduction of EcoOl09I Methyltransferase(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
In order to characterize EcoO109I methyltransferase, a recombinant Escherichia coli clone that overproduces the enzyme was constructed. The coding region of M.EcoO109I was joined to the lac promoter of an expression vector, pUC118, and the resulting plasmid was introduced into E. coli HB101. M.EcoOW9I was purified homogeneously from IPTG-induced cells, and was found to consist of a monomer subunit. M.Eco109I uniquely methylates the inner deoxycytidylate residue in the sequence 5'-(A/G)GGNCC(C/T)-3' to produce 5-methylcytosine. The enzyme was most active at pH 8.0-8.5 and 50℃. The enzyme activity was not affected by the addition of Mg^<2+> or EDTA.
- 社団法人日本農芸化学会の論文
- 2001-11-23
著者
-
KITA Keiko
Department of Biotechnology, Faculty of Engineering, Tottori University
-
Kita Keiko
Department Of Biotechnology Faculty Of Engineering Tottori University
-
Kita Keiko
Department Of Biotechnology Tottori University
-
TSUDA Junko
Department of Biotechnology, Tottori University
-
NISHIGAKI Ryu-ichi
Department of Biotechnology, Tottori University
-
Tsuda Junko
Department Of Biotechnology Tottori University
-
Nishigaki Ryu-ichi
Department Of Biotechnology Tottori University
関連論文
- Isolation and Primary Structural Analysis of Two Conjugated Polyketone Reductases from Candida parapsilosis(Biochemistry & Molecular Biology)
- Diversity of 4-Chloroacetoacetate Ethyl Ester-Reducing Enzymes in Yeasts and Their Application to Chiral Alcohol Synthesis
- Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
- Microbial Resolution of 1-Phenoxy-2-propanol by Stereospecific Decomposition and Asymmetric Hydrolysis of 1-Phenoxy-2-propyl Acetate
- Expression of the Extracellular Levansucrase and Invertase Genes from Zymomonas mobilis in Escherichia coli Cells
- Purificatioin, Crystallization, and Characterization of the Extracellular Invertase from Zymomonas mobilis
- Cloning, Sequence Analysis, and Expression of the Gene Encoding Formaldehyde Dismutase from Pseudomonas putida F61
- Preparation of [1-^C]D-Glucose 6-Phosphate from [^C]Methanol and D-Ribose 5-Phosphate with Methylotrophic Enzymes
- Purification and Properties of Aldehyde Reductases from Yeasts That Convert Ethyl 2-Acetamido-3-oxobutyrate to Optically Active Ethyl 2-Acetamido-3-hydroxybutyrate
- Degradation of Phenols by Thermophilic and Halophilic Bacteria Isolated from a Marine Brine Sample
- Purification, Crystallization, and Properties of the Extracellular Levansucrase from Zymomonas mobilis
- Pyruvate Production by Enterococcus casseliflavus A-12 from Gluconate in an Alkaline Medium
- Production of D-[1-^C]Fructose 6-Phosphate by a Formaldehyde-fixing System in Methylomonas aminofaciens 77a
- Site-Specific Restriction Endonucleases of Several Non-Pathogenic Bacteria
- Purification and Characterization of Sequence-Specific Restriction Endonuclease MflI
- Characterization and Overproduction of EcoOl09I Methyltransferase(Biochemistry & Molecular Biology)
- Solvent Extraction of Thallium(I) with Chelating Extractants Coordinating through Oxygen Atoms