Site-Specific Restriction Endonucleases of Several Non-Pathogenic Bacteria

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概要

• 論文の詳細を見る

• Seventy-one non-pathogenic bacterial strains were surveyed for the presence of Type II restriction endonucleases in aerobic culture. Five strains were found to contain specific enzymes : Cfl I from Cellulomonas flavigena, Gal I from Gluconobacter albidus, Gce I from Gluconobacter cerinus, Hac I from Halococcus acetoinfaciens, and Mfl I from Microbacterium flavum. These enzymes cleaved respectively the sequences of 5'-CTGCA↓G-3', 5'-CCGC↓GG-3', 5'-CCGC↓GG-3', 5'-↓GATC-3', and 5'-Pu↓GATCPy-3' at the positions indicated. Hac I and Mfl I digested DNA from the Escherichia coli Dam^- strain but not from the Dam^+ strain, indicating that they react only with unmodified sequences. With NaCl or KCl, the optimal salt concentrations were 40-60 mM for Cfl I and 175-300 mM for Hac I; Gal I and Gce I did not require either salts. Activity was greatest at pH 7.1-7.5 for Cfl I and Hac I, and at pH 7.5-8.0 for Gal I and Gce I.

• 社団法人日本生物工学会の論文
• 1985-04-25

著者

• KITA Keiko

  Department of Biotechnology, Faculty of Engineering, Tottori University

• KITA KEIKO

  Central Research Laboratories, Takara Shuzo Co. Ltd.

• HIRAOKA NOBUTSUGU

  Central Research Laboratories, Takara Shuzo Co. Ltd.

• NAKAJIMA Hiroshi

  central Research Institute, Maruha Co., Ltd.

• Kita K

  Tottori Univ. Tottori Jpn

• Kita Keiko

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Kita Keiko

  Central Research Laboratories Takara Shuzo Co. Ltd.

• Kita Keiko

  Department Of Food Technology Faculty Of Engineering Fukuyama University

• Nakajima H

  Division Of Dental Biomaterials Science Department Of Restorative And Biomaterials Sciences Meikai U

• Hiraoka N

  Alcoholic Beverages Research Laboratories Takara Shuzo Co.ltd.

• Hiraoka Nobutsugu

  Central Research Laboratories Takara Shuzo Co. Ltd.

• KIMIZUKA FUSAO

  Central Research Laboratories, Takara Shuzo Co., Ltd.,

• OBAYASHI AKIRA

  Central Research Laboratories, Takara Shuzo Co., Ltd.,

• Kimizuka F

  Takara Shuzo Co. Ltd. Shiga Jpn

• Obayashi A

  Central Research Laboratories Takara Shuzo Co. Ltd.

• KIMIZUKA Fusao

  Central Research Laboratories, Takara Shuzo Co., Ltd.

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