Enzymatic Production of Fructose 1, 6-Diphosphate Using Crude Cell Extract of Bacillus stearothermophilus

概要

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The enzymatic production of fructose 1, 6-diphosphate(FDP)from glucose was performed in a batch reactor and a semibatch reactor using the crude cell extract of Bacillus stearothermophilus which contains all four enzymes required for the synthesis. The experimental results of the yield and the time courses of FDP production obtained using various enzyme concentrations were in good agreement with the theoretical predictions calculated based on the differential equations including the rate equations of the four enzymes, which were determined using the purified enzymes of B.stearothermophilus.
社団法人日本生物工学会の論文
1999-05-25