Experimental Investigation of Fructose 1, 6-Diphosphate Production and Simultaneous ATP Regeneration by Conjugated Enzymes in an Ultrafiltration Hollow-Fiber Reactor

概要

論文の詳細を見る
The enzymatic synthesis of fructose 1, 6-diphosphate (FDP) from glucose and the enzymatic ATP regeneration were performed simultaneously in an ultrafiltration hollow-fiber reactor using both the purified enzymes and the crude cell extract of Bacillus stearothermophilus. The process consisted of the three-step synthetic reactions catalyzed by glucokinase, phosphoglucose isomerase and phosphofructokinase, and the ATP regeneration reaction catalyzed by acetate kinase. The experimental results of the yield and the recycle number agreed well with the theoretical predictions calculated using a computer program developed in our preceding study. This proved the validity of this computer program for predicting or analyzing the reactor performance.
社団法人日本生物工学会の論文
1999-12-25