Characterization of α-Ketog1utarate-Resistant Sake Yeast Mutant with High Organic Acid Productivity
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概要
- 論文の詳細を見る
A yeast with high organic acid productivity was isolated from α-ketoglutarate-resistant mutants obtained by mutagenizing a sake yeast, Kyokai no. 701 (K-701). The new strain, 20G-R39, produces about twice as much malate and succinate as the parental strain. DNA microarray analyses revealed that the transcriptional levels of genes involved in the TCA cycle, oxidative phosphorylation, and respiration were higher in strain 20G-R39 than in strain K-701. Expression of these genes is regulated by the Hap2/3/4/5p complex, and especially by expression of the HAP4 gene. In a Northern blot analysis, the transcriptional level of the HAP4 gene was higher in strain 20G-R39 than in strain K-701. We constructed a plasmid that expresses the HAP4 gene constitutively and introduced it into strain K-701. The HAP4-overexpression-strain produced more malate and succinate than strain K-701 both in YPD medium and in a sake brewing test. These results indicate that strain 20G-R39 produces more organic acids than strain K-701 because strain 20G-R39 has a higher level of expression of the HAP4 gene than strain K-701.
- 社団法人日本生物工学会の論文
- 2003-10-25
著者
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YANO Shigekazu
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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SHIMOI Hitoshi
National Research Institute of Brewing
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Shimoi H
Hiroshima Univ. Higashihiroshima Jpn
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Shimoi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shimoi Hitoshi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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ITO KIYOSHI
National Research Institute of Brewing
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Hiramatsu Junichi
Alcoholic Beverages And Foods Research Laboratories Takara Shuzo Co. Ltd.
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YANO SHUNTARO
Alcoholic Beverages and Foods Research Laboratories, Takara Shuzo Co. Ltd.
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ASANO TADAO
Alcoholic Beverages and Foods Research Laboratories, Takara Shuzo Co. Ltd.
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KUROSE NAOTAKA
Alcoholic Beverages and Foods Research Laboratories, Takara Shuzo Co. Ltd.
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Yano Shuntaro
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Kurose N
Alcoholic Beverages And Foods Research Laboratories Takara Shuzo Co. Ltd.
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Asano Tadao
Alcoholic Beverages And Foods Research Laboratories Takara Shuzo Co. Ltd.
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