High Expression of Unsaturated Fatty Acid Synthesis Gene OLE1 in Sake Yeasts(Brewing and Food Technology)
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概要
- 論文の詳細を見る
GeneFilters^[○!R] and Northern blot analysis revealed that the sake yeast strain Kyokai no.7 (K7) showed a higher expression level of OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using OLE1 promoter: lacZ fusion reporter genes revealed that both the cis element of the OLE1 promoter and trans factors are involved in the increased expression of OLE1 in sake yeasts.
- 社団法人日本生物工学会の論文
- 2005-05-25
著者
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Yamada T
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Yamada Tasuku
General Res. Lab. Of Kiku-masamune Sake Brewing Co. Ltd.
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Yamada Tasuku
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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SHIMOI Hitoshi
National Research Institute of Brewing
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Shimoi H
Hiroshima Univ. Higashihiroshima Jpn
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Shimoi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shimoi Hitoshi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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ITO KIYOSHI
National Research Institute of Brewing
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