Flocculation Mechanism of Hansenula anomala J224(Microbiology & Fermentation Industry)

概要

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The flocculation mechanism of a flocculent yeast, Hansenula anomala J224, isolated from a waste water treatment tank of a Japanese sake brewing factory, was investigated by comparing some characteristics with strain J224-1 (a nonflocculent mutant of strain J224). The cell titration curves showed that strain J224 was different from strain J 224-1 in charge distribution on the cell surface. The cell wall of strain J224 was more abundant in Mg^<2+>, Ca^<2+>, Na^+, NH^4_+ , and total phosphorus contents but had less fatty acids than that of strain J 224-1. The cell walls of both strains had almost the same amino acid composition. Acylation of strain J224 with acetic anhydride caused deflocculation. By treatment with proteinase K, an additional protein of 37 K dalton (37 K-protein) whose molecular mass was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was released in the supernatant from strain J224 cells. The 37 K-protein was purified by cation-exchange high-performance liquid chromatography. The amino acid composition of this protein was different from that of the cell wall. Flocculation of strain J224 was promoted by the addition of 37 K-protein. We discussed the involvement of ionic interaction between 37 K-protein and phosphate and hydrophobic interaction between 37 K-proteins on the cell surface of adjacent cells in the flocculation of strain J224.
社団法人日本農芸化学会の論文
1990-06-23