Disruption of ubiquitin-related genes in laboratory yeast strains enhances ethanol production during sake brewing(BREWING AND FOOD TECHNOLOGY)
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概要
- 論文の詳細を見る
Sake yeast can produce high levels of ethanol in concentrated rice mash. While both sake and laboratory yeast strains belong to the species Saccharomyces cerevisiae, the laboratory strains produce much less ethanol. This disparity in fermentation activity may be due to the strains' different responses to environmental stresses, including ethanol accumulation. To obtain more insight into the stress response of yeast cells under sake brewing conditions, we carried out small-scale sake brewing tests using laboratory yeast strains disrupted in specific stress-related genes. Surprisingly, yeast strains with disrupted ubiquitin-related genes produced more ethanol than the parental strain during sake brewing. The elevated fermentation ability conferred by disruption of the ubiquitin-coding gene UBI4 was confined to laboratory strains, and the ubi4 disruptant of a sake yeast strain did not demonstrate a comparable increase in ethanol production. These findings suggest different roles for ubiquitin in sake and laboratory yeast strains.
著者
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Kitagaki Hiroshi
National Research Institute of Brewing
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Akao Takeshi
National Research Institute of Brewing
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Wu Hong
National Research Institute Of Brewing
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SHIMOI Hitoshi
National Research Institute of Brewing
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Watanabe Tomoko
National Research Institute of Brewing
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Araki Yoshio
National Research Institute of Brewing
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Takagi Hiroshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Shimoi H
Hiroshima Univ. Higashihiroshima Jpn
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Shimoi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shimoi Hitoshi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Takagi Hiroshi
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Takagi Hiroshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Watanabe Tomoko
National Research Institute Of Brewing:graduate School Of Advanced Sciences Of Matter Hiroshima Univ
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