Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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We characterized the yeast γ-glutamyl kinase important for proline biosynthesis. Asp154 and Ile150 were involved in the affinity with glutamate as well as proline. The Asp154Asn and Ile150Thr mutants had increased thermostability, in addition to desensitization to proline. Deletion of a C-terminal domain dramatically decreased the reaction rate and stability.
著者
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Takagi Hiroshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Takagi Hiroshi
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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TATEHASHI Yuki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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