Functional Analysis of Genes Encoding Putative Oxidoreductases in Aspergillus oryzae, Which Are Similar to Fungal Fructosyl-Amino Acid Oxidase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
We found 11 genes (FAO1-11) encoding putative oxidoreductases in the Aspergillus oryzae genome, which are similar to fungal fructosyl-amino acid oxidases. The cDNAs corresponding to the genes were cloned and expressed in Escherichia coli. rFao2 had fructosyl-amino acid oxidase activity, whereas rFao1 did not show any enzyme activity, even though the deduced amino acid sequence of Fao1 is identical to that of one of the fructosyl-amino acid oxidase isozymes from Aspergillus oryzae. rFao7 and rFao8 showed oxidase activity toward sarcosine, L-pipecolate, and L-proline. rFao10 was active toward only sarcosine, of the substrates tested. The functions of the other proteins were also predicted from a phylogenetic analysis.
- 社団法人日本生物工学会の論文
- 2007-11-25
著者
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Ishida Hiroki
Gekkeikan Sake Co. Ltd.
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Ishida Hiroki
Research Institute Gekkeikan Sake Co. Ltd.
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Yoshida N
Miyazaki Univ. Miyazaki Jpn
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Yoshida Naoto
Department Of Biological Resource Science Miyazaki University
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Takagi Hiroshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Yoshida Nobuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Takagi Hiroshi
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Tani Yoshiki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Akazawa Shin-ichi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Karino Tetsuya
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Kanagawa T
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Karino Tetsuya
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Takagi Hiroshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Tani Yoshiki
Graduate School Of Biological Sciences Advanced Institute Of Science And Technology
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Akazawa Shin-ichi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Ishida Hiroki
Research Institute, Gekkeikan Sake Co., Ltd.
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