Mutations within the Reactive-site Region of Human Pancreatic Secretory Trypsin Inhibitor Confer α-Thrombin and Factor Xa Inhibitory Activities

概要

論文の詳細を見る
Mutations were introduced into the reactive-site region of human pancreatic secretory trypsin inhibitor (PSTI) to produce thrombin and/or factor Xa inhibitors. All of five mutants showed trypsin inhibitory activity as strong as wild-type PSTI. Moreover, the Arg (P1), Pro-Arg (P2-P1), and Pro-Arg-Ile-Tyr-Asn (P2-P1-P1'-P2'-P3') (bold letters indicate replaced amino acids compared to the wild type) mutants had additional inhibitory activities toward factor Xa, both thrombin and factor Xa, and thrombin, respectively, at 1×10^<-5>M.
社団法人日本農芸化学会の論文
1997-02-23