Expression and Characterization of Rat Pancreatic Secretory Trypsin Inhibitor-I and -II in Saccharomyces cerevisiae, and Simple Purification by Affinity Chromatography
スポンサーリンク
概要
- 論文の詳細を見る
Rat pancreatic secretory trypsin inhibitor-I (PSTI-I) and -II (PSTI-II) were expressed in Saccharomyces cerevisiae AH22 under the control of the PHO5 promoter. Both proteins were secreted into the culture broth and purified by affinity chromatography using a genetically engineered mutant Streptomyces fradiae trypsin-like enzyme as a ligand. Rat PSTI-I was purified to homogeneity by one-step affinity chromatography, but the PSTI-II fraction contained two molecular mass forms (6.2 kDa and 5.6 kDa) after affinity chromatography. N-terminal amino acid sequencing revealed that the larger molecular mass protein was intact rat PSTI-II and five amino acid residues at its N-terminal had been truncated to the smaller form. The N-terminal amino acid sequences, amino acid compositions and trypsin inhibitory activities of recombinant rat PSTI-I and -II were identical to those of natural PSTIs. Moreover, the pancreatic exocrine secretion activity of recombinant rat PSTI-I in vivo also agreed well with that of natural PSTI-I.
- 社団法人日本生物工学会の論文
- 1996-11-25
著者
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FUNAKOSHI Akihiro
Department of Gastroenterology, National Kyushu Cancer Center
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Yoshida N
Miyazaki Univ. Miyazaki Jpn
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Yoshida Naoto
Department Of Biological Resource Science Miyazaki University
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Nagata K
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Miyasaka K
Tokyo Metropolitan Inst. Gerontology Tokyo Jpn
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Miyasaka Kyoko
Tokyo Metropolitan Institute Of Gerontology
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KATOH TAKAAKI
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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HORII TOSHIHIKO
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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FUJIWARA TAKASHI
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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KIKUCHI NORIHISA
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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SHIN MASARU
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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NAGATA KIYOSHI
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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YOSHIDA NOBUO
Shionogi Research Laboratories, Shionogi & Co. Ltd.,
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MIYASAKA KYOKO
First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology
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Funakoshi A
National Kyushu Cancer Center Fukuoka Jpn
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Funakoshi Akihiro
Department Of Digestive Diseases National Kyushu Cancer Center
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Yoshida Nobuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Nagata Kiyoshi
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Shin Masaru
Department Of Urology Osaka University Graduate School Of Medicine
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Shin Masaru
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Katoh Takaaki
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Horii Toshihiko
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Miyasaka K
Tokyo Metropolitan Institute Of Gerontology
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Kikuchi N
Shionogi Hyogo Jpn
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Miyasaka Kyoko
Dep. Of Clinical Physiology Tokyo Metropolitan Inst. Of Gerontology Jpn
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Fujiwara Takashi
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Kikuchi Norihisa
Shionogi Research Laboratories Shionogi & Co. Ltd.
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SHIN Masaru
Shionogi Laboratories, Shionogi and Co., Ltd.
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