Characterization of a Flavinogenic Mutant of Methanol Yeast Candida boidinii and Its Extracellular Secretion of Riboflavin
スポンサーリンク
概要
- 論文の詳細を見る
A flavinogenic mutant was derived from Candida boidinii by mutagenesis. The mutant was smaller than the wild type, did not grow on a minimal medium, and required L-tryptophan, L-leucine, inositol, and nicotinate for growth. The mutant was defective in the oxidative pentose phosphate pathway, lacking glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The specific activities of the transaldolase and transketolase of the mutant were higher than those of the wild type. These high activities might direct the flux of the carbon source to the nonoxidative pathway with formation of a large amount of pentose phosphates, increasing riboflavin synthesis. Under microaerobic conditions at 25℃, 90mg/l riboflavin was obtained.
- 2000-07-25
著者
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Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Yoshida N
Miyazaki Univ. Miyazaki Jpn
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Yoshida Naoto
Department Of Biological Resource Science Miyazaki University
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Yoshida Nobuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Laboratory Of Cell Biotechnology Graduate School Of Biological Sciences Nara Institute Of Science An
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Suryadi Herman
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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SURYADI HERMAN
Laboratory of Cell Biotechnology, Graduate School of Biological Sciences, Nara Institute of Science
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YOSHIDA NOBUYUKI
Laboratory of Cell Biotechnology, Graduate School of Biological Sciences, Nara Institute of Science
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YAMADA-ONODERA KEIKO
Laboratory of Cell Biotechnology, Graduate School of Biological Sciences, Nara Institute of Science
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TANI YOSHIKI
Laboratory of Cell Biotechnology, Graduate School of Biological Sciences, Nara Institute of Science
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Kanagawa T
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Yamada-onodera Keiko
Laboratory Of Cell Biotechnology Graduate School Of Biological Sciences Nara Institute Of Science An
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Tani Yoshiki
Laboratory Of Cell Biotechnology Graduate School Of Biological Sciences Nara Institute Of Science An
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