Effects of Organic Solvents and Chemical Modification on the Activity of Glycerol Dehydrogenase(Microbiology & Fermentation Industry)
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概要
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The addition of an organic solvent to the reaction mixture led to a change in the activity of glycerol dehydrogenase. The activity on glycerol decreased to lower than that on 1,2-propanediol. This was due to the apparent increase in the Km value for each substrate. The system was applied to determination of 1,2-propanediol, The standard curve for 1,2-propanediol with a rate assay method was not affected by a 10-fold amount of glycerol in the presence of n-butanol. Chemical modification, except for succinylation of the NH_2-residue, did not cause any change in substrate specificity. The participation of a His-residue in the active site was suggested by the results of chemical modification.
- 1987-12-23
著者
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NISHISE Hiroshi
Department of Agricultural Chemistry, Kyoto University
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Nishise H
Koshien Univ.
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Maehashi Susumu
Department Of Agricultural Chemistry Kyoto University
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