Raw Cassava Starch-Digestive Glucoamylase of Aspergillus sp. N-2 Isolated from Cassava Chips

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概要

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• One hundred and eighty strains of black aspergilli isolated from cassava fields and factories in Thailand were screened for the activity of raw cassava starch-digestive glucoamylase. Aspergillus sp. N-2 was selected as the best producer and its extracellular glucoamylase production was investigated. Conditions for the production were optimized for both liquid and solid cultures, and solid culture and found to be approximately three times more efficient than liquid culture. The culture filtrate showed strong glucoamylase activity at low pH (pH 2.0) and high temperature (55℃), and could digest high concentration raw cassava starch. The glucoamylase activity was separated to four fractions (A, B, C and D) by DEAE-Sephacel column chromatography. Fraction C was obtained in a homogeneous state with a molecular weight of 92,000. Each fraction was characterized in terms of the properties of the glucoamylase activity and the efficiency of digestion of cooked and raw cassava starch.

• 社団法人日本生物工学会の論文
• 1986-10-25

著者

• Kumnuanta Jaroon

  Central Laboratory And Greenhouse Complex Kamphaengsaen Campus Kasetsart University

• TANI Yoshiki

  Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• VONGSUVANLERT VITCHUPORN

  Central Laboratory and Greenhouse Complex, Kamphaengsaen Campus, Kasetsart University

• Vongsuvanlert Vitchuporn

  Central Laboratory And Greenhouse Complex Kamphaengsaen Campus Kasetsart University

• Tani Yoshiki

  Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University

• Tani Yoshiki

  Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University

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