Bioconversion of Dinitrile to Mononitrile, a Tranexamic Acid Intermediate, by Corynebacterium sp.(Microbiology & Fermentation Industry)

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An isolated bacterial strain, Corynebacterium sp. C5, could produce trans-4-cyanocyclohexane-1-carboxylic acid (t-MCC), an intermediate in the chemical synthesis of tranexamic acid, from trans-1,4-dicyanocyclohexane (t-DCC), through catalysis by two enzymes, nitrile hydratase and amidase. The two enzymes were constitutively formed in cells. The activity of nitrile hydratase increased with the addition of FeSO_4 and isobutyronitrile to the culture medium. The activity of amidase increased with the addition of isobutyronitrile. Peptone, as the nitrogen source, was effective for production of the two enzymes without a decrease in their activities in the stationary growth phase. The cells could produce 92.1mg/ml t-MCC from 100mg/ml t-DCC on 20-hr incubation in a resting cell system.
社団法人日本農芸化学会の論文
1989-12-23