Characterization of D-Sorbitol Dehydrogenase Involved in D-Sorbitol Production of a Methanol Yeast, Candida boidinii (Kloeckera sp.) No.2201(Microbiology & Fermentation Industry)
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概要
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D-Sorbitol (sorbitol) production from D-glucose could be achieved by initial isomerization of D-glucose to D-fructose via xylose isomerase, then reduction to sorbitol via sorbitol dehydrogenase coupled with NADH regeneration from the methanol oxidative enzymes system by an intact cell system of a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201. Sorbitol dehydrogenase was purified 351-fold from a cell-free extract of D-xylose-grown cells and characterized. It oxidized preferably D-mannitol besides sorbitol, and reduced preferably D-xylulose and D-ribulose, besides D-fructose. Km values for sorbitol and D-fructose were 7.7 and 263mM, and V_<max> values were 301 and 384 μmol/min/mg, respectively. The enzyme activity was strongly stimulated by Fe^<3+> and significantly inhibited by Pb^<2+>, EDTA, and o-phenanthroline.
- 社団法人日本農芸化学会の論文
- 1988-02-23
著者
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Vongsuvanlert Vitchuporn
Research Center For Cell And Tissue Culture Faculty Of Agriculture
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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