Sorbitol Production by a Methanol Yeast, Candida boidinii (Kloeckera sp.) No. 2201

概要

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One hundred and eleven strains of methanol yeast were screened for their sorbitol productivity from glucose using an intact cell system in which methanol was used as the energy source for regenerating NADH for the reduction of glucose to sorbitol. Candida boidinii (Kloeckera sp.) no. 2201 was selected as the best strain and investigated with respect to sorbitol production.Cells having high sorbitol-productivity were prepared by cultivating the yeast in a medium containing methanol and xylose. The reaction mixture for sorbitol production consisted of 0.5 M methanol, 92.5 mM glucose, 16.6 mM Tris-HCl (pH 8.0), 100 mM Fe^<2+>, 0.5% PEG-4000 and intact cells (15 mg as dry cell weight) in a total volume of 9 ml. The highest value of sorbitol production was obtained by incubation of the reaction mixture under shaking at 37℃.The maximum amounts of D-sorbitol, 8.8 g/l and 19.1 g/l, were obtained from 20 g/l of D-glucose and D-fructose, respectively. L-Iditol, 20 g/l, was also obtained by complete conversion from L-sorbose.
1987-08-25