Biotin Overproduction by Biotin Analog-resistant Mutants of Bacillus sphaericus

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概要

• 論文の詳細を見る

• Bacillus sphaericus produced larger amounts of biotin when its precursor was added to the mediumafter 24-hr cultivation than when it was added before cultivation. Actithiazic acid (ACM)-and/or 5-(2-thienyl)-valeric acid (TVA)-resistant mutants were induced from B. sphaericus by treatment with N-methyl-N-nitro-N-nitrosoguanidine. ACM-and TVA-resistant mutant, AB12, excreted 7. 1 times more biotin than the wild type strain. The TVA-resistant mutant, A 16, excreted 1 1.9 times more total biotin than the wild type strain. The reaction conditions for biotin production using resting cells of strain AB12 were investigated. After 48-hr reaction, strain AB12 accumulated 9.5μg/ml of biotin under the optimum conditions.

著者

• YAMADA Hideaki

  Department of Agricultural Chemistry, Kyoto University

• Izumi Yoshikazu

  Department Of Agricultural Chemistry Kyoto University

• Tani Yoshiki

  Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University

• Yamada Hideaki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• OSAKAI Miyako

  Department of Agricultural Chemistry, Kyoto University

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