Biotin Overproduction by Biotin Analog-resistant Mutants of Bacillus sphaericus
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概要
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Bacillus sphaericus produced larger amounts of biotin when its precursor was added to the mediumafter 24-hr cultivation than when it was added before cultivation. Actithiazic acid (ACM)-and/or 5-(2-thienyl)-valeric acid (TVA)-resistant mutants were induced from B. sphaericus by treatment with N-methyl-N-nitro-N-nitrosoguanidine. ACM-and TVA-resistant mutant, AB12, excreted 7. 1 times more biotin than the wild type strain. The TVA-resistant mutant, A 16, excreted 1 1.9 times more total biotin than the wild type strain. The reaction conditions for biotin production using resting cells of strain AB12 were investigated. After 48-hr reaction, strain AB12 accumulated 9.5μg/ml of biotin under the optimum conditions.
著者
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YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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OSAKAI Miyako
Department of Agricultural Chemistry, Kyoto University
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