Effect of Intracellular Concentration of Vitamin B6 on Tryptophanase Activity in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
The intracellular concentration of vitamin B6 (B6) of a wild type strain (WG1) of Escherichia coli B remained almost constant (0.25 to 0.35 nmol per mg cells) when different amounts ofB6 were extracellularly added. However, B6-requiring mutants were strongly affected by extracellular B6. Activities of tryptophanase in a B6-requiring mutant, strain WG3, grown under various conditions were measured. A clear correlation between intracellular B6concentration and the ratio of holo-tryptophanase activity (i.e., holo-activity/total activity) was observed. Tryptophanase of strain WG3 grown under B6-deficient conditions and the enzyme of strain WG1 were purified and their properties compared. The purified enzymes of both strains WG3 and WG1 showedsimilar characteristics, but a difference was observed in the antigen activities.
- 社団法人 日本農芸化学会の論文
著者
-
MATSUURA Motohiro
Department of Physics, Faculty of Science, Kyoto University
-
YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
-
Yamada H
Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University
-
TANI Yoshiki
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
-
KARAI Nobuyuki
Department of Agricultural Chemistry, Kyoto University
-
Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
-
Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
-
MATSUURA Motohiro
Department of Agricultural Chemistry, Kyoto University
関連論文
- N.M.R. Study of Magnetic Ordering in Two-Dimensional Antiferromagnet, Mn(HCOO)_2・2H_2O
- Magnetic Phase Transition of Antiferromagnet with Weak Canting Interaction
- A Field Induced Antiferromagnetic Order in Cu (HCOO)_2・4D_2O above T_N
- A Processing Technique for Cell Surfaces Using Gas Cluster Ions for Imaging Mass Spectrometry
- Stimulatory Effect of Peanut Oil on the Production of Dihomo-γ-linolenic Acid by Filamentous Fungi(Microbiology & Fermentation Industry)
- Production of Arachidonic Acid by Mortierella elongata 1S-5(Microbiology & Fermentation Industry)
- Distribution of Threonine Aldolase Activity with Different Stereospecificities in Aerobic Bacteria
- Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
- Enzymes Involved in the NADPH Regeneration System Coupled with Asymmetric Reduction of Carbonyl Compounds in Microorganisms
- Novel Enzymatic Production of D-(-)-Pantoyl Lactone through the Stereospecific Reduction of Ketopantoic Acid(Microbiology & Fermentation Industry)
- A New Enzymatic Method for the Measurement of Creatinine Involving a Novel ATP-dependent Enzyme, N-Methylhydantoin Amidohydrolase
- Nucleoside-Triphosphatase Activity of an ATP-Dependent Enzyme, N-Methylhydantoin Amidohydrolase
- Occurrence of Amidases in the Industrial Microbe Rhodococcus rhodochrous J1
- Identification of the Female Sex Pheromone of the Cabbage Webworm
- Effects of Organic Solvents and Chemical Modification on the Activity of Glycerol Dehydrogenase(Microbiology & Fermentation Industry)
- Purification, Crystallization and Properties of Hydantoinase from Pseudomonas striata : Microbial Transformation of Hydantoins to Amino Acids(II)
- Distribution of Hydantoin Hydrolyzing Activity in Microorganisms : Microbial Transformation of Hydantoins to Amino Acids(I)
- Characterization of Nitrile Hydratase and Amidase, Which Are Responsible for the Conversion of Dinitriles to Mononitriles, from Corynebacterium sp.(Microbiology & Fermentation Industry)
- Bioconversion of Dinitrile to Mononitrile, a Tranexamic Acid Intermediate, by Corynebacterium sp.(Microbiology & Fermentation Industry)
- Formaldehyde Production with Heat-Treated Cells of Methanol Yeast
- Production of Formaldehyde by a Mutant of Methanol Yeast, Candida boidinii S2
- Formaldehyde Production by Cells of a Mutant of Candida boidinii S2 Grown in Methanol-limited Chemostat Culture(Microbiology & Fermentation Industry)
- Simple Purification and Improvement of Stability of Alcohol Oxidase from a Methanol Yeast, Candida boidinii S2, by Forming an Enzyme-Azide Complex(Microbiology & Fermentation Industry)
- 416 Methyl formate production by methanol-utilizing yeast, Pichia methanolica (P. aganobii)
- S-Adenosyl-L-methionine Production by Saccharomyces sake : Optimization of the Culture Conditions for the Production of Cells with a High S-Adenosyl-L-methionine Content(Microbiology & Fermentation Industry)
- Raw Cassava Starch-Digestive Glucoamylase of Aspergillus sp. N-2 Isolated from Cassava Chips
- Self-assembly and magnetic actuation of three dimensional micro hinged mechanisms (特集 センサ・マイクロマシン作製における自己組織化)
- Enzymes Involved in Theobromine Production from Caffeine by Pseudomonas putida No.352
- Microbial Production of Theobromine from Caffeine
- Purification and Characterization of Methanol Dehydrogenase of a Serine-Producing Methylotroph, Hyphomicrobium methylovorum
- Photoproduction of Hydrogen by Immobilized Cells of a Photosynthetic Bacterium, Rhodospirillum rubrum G-9 BM
- Threonine Production from Glycine and Ethanol by a Methanol-Utilizing Bacterium
- Synthesis of D-Cysteine from a Racemate of 3-Chloroalanine by Phenylhydrazine-treated Cells of Pseudomonas putida CR 1-1
- Identification of Reaction Products of Polyethylene Glycol Dehydrogenase
- Cofactor Requirements of Constitutive Aromatic L-AminoAcid Decarboxylase from Micrococcus percitreus
- Effect of Intracellular Concentration of Vitamin B6 on Tryptophanase Activity in Escherichia coli
- A New Intact Cell System for Determination of the Rate of Vitamin B6 Biosynthesis
- Glycerol Dehydrogenase from Cellulomonas sp. NT3060: Purification and Characterization
- Formation of Glycerol Dehydrogenase by Microorganismst
- The Assembly of a Major Outer Membrane Protein (OmpF) in the Cell Surface of Escherichia coli
- Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
- Biotin Biosynthesis in Microorganisms (Commemoration Issue Dedicated to Professor Tatsuo Yamamoto on the Occasion of his Retirement)
- Cloning, Sequencing, and Heterologous Expression of a Gene Coding for Arthromyces ramosus Peroxidase
- An Obligate Methylotrophic Hyphomicrobium Strain Identification, Growth Characteristics and Cell Composition
- L-Serine Production by Resting Cells of a Methanol-Utilizing Bacterium
- Crystallization and Characterization of Sarcosine Oxidase from Alcaligenes denitrificans subsp. denitrificans(Biological Chemistry)
- Sarcosine Oxidase Involved in Creatinine Degradation in Alcaligenes denitrificans subsp. denitrificans J9 and Arthrobacter spp. J5 and J11(Microbiology & Fermentaion Industry)
- A New Method for 5-(4-Hydroxyphenyl)hydantoin Synthesis
- Production of Pyranose Oxidase by Basidiomycetous Fungus No. 52
- Purification and Properties of Pyranose Oxidase from Basidiomycetous Fungus No. 52(Microbiology & Fermentation Industry)
- Isolation of a New Pyranose Oxidase Producing Basidiomycete(Microbiology & Fermentation Industry)
- Intergrain Ordering of a Superconductive Ceramic of YBa_2Cu_4O_8 at Zero External Magnetic Field Studied by Linear and Nonlinear Transport Coefficients
- L-Iditol Production from L-Sorbose by a Methanol Yeast, Candida boidinii (Kloeckera sp.) No. 2201
- Sorbitol Production by a Methanol Yeast, Candida boidinii (Kloeckera sp.) No. 2201
- Pulsed Adiabatic-Magnetization in Cu(NO_3)_2・2.5H_2O
- Negative Divergence of Nonlinear Susceptibility at the Intergrain Phase Ordering Transition in a Superconductive Ceramic of YBa_2Cu_4O_8
- Successive Phase Transitions of a Hierarchical Nature in a YBa_2Cu_4O_8 Ceramic Superconductor
- Spontaneous Subsystem Magnetizations of a Heterogeneous Layer Antiferromagnet Mn (HCOO)_2.2D_2O
- Separate Observation of Subsystem Susceptibilities in Mn (HCOO)_2・2D_2O by Proton NMR
- Nuclear Magnetic Resonances of Mn^ and N^ in Ferrimagnetic Intermetallic Mn_4N -- Anisotropic and Transferred Hyperfine Fields in Intermetallic Compound
- Nuclear Magnetic Resonances of Fe^ in Fe_4N and Mn^ in Mn_4N
- Nuclear Magnetic Resonance of Fe^ in Nickel and Nickel-Zinc-Ferrites
- Acid Phosphatase/Phosphotransferases from Enteric Bacteria
- A Novel Selective Nucleoside Phosphorylating Enzyme from Morganella morganii
- Successive Transitions in CoCl_2-Graphite Intercalation Compound snd the Stage Dependence
- Nonlinear Slow Fluctuation in Magnetic Ordering of CoCl_2-GIC -Observation of a 1/ω-Type Spectrum-
- Asymmetric Critical Phenomena in a Quasi Two-Dimensional-Heisenberg Antiferromagnet Mn (HCOO)_2.2H_2O
- Novel Chemoenzymatic Syntesis of D(-)-Pantoyl Lactone(Microbiology & Fermentation Industry)
- Networking and Cooperative Dynamics in Complex Physical Systems (II)
- Symmetry Breaking and Nonlinear Susceptibility in a Complex Physical System,CoCl_2-GIC
- Nuclear Magnetic Resonance of Fe^ in α^Fe_2O_3
- Hyperfine Field at Fe^ in Lithium Ferrite Measured by NMR.
- Magnetic Phase Transition in Ni_xCo_Cl_2・6H_2O
- Magnetic Phase Transition for Dilute Heisenberg Spin System in Two-Dimensional Lattice
- Novel 4RTD Logic Circuits(Nanomaterials and Quantum-Effect Devices, Fundamental and Application of Advanced Semiconductor Devices)
- Isolation of Advantageous Mutants of Pseudomonas chlororaphis B23 for the Enzymatic Production of Acrylamide(Microbilolgy & Fermentation Industry)
- Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase(Microbiology & Fermentaion Industry)
- Biochemical Characterization of an L-Methionine-Enriched Mutant of a Methylotrophic Yeast, Candida boidinii
- Production of Raw Cassave Starch-Digestive Glusoamylase by Rhizopus sp. in Liquid Culture
- An Assay for Serine-glyoxylate Aminotransferase(Microbiology & Fermentation Industry)
- Proton Nuclear Magnetic Resonance Study in Manganese Formate Dihydrate
- Dihydroxyacetone Reductase Responsible for Less-aerobic Growth of a Methylotrophic Yeast, Hansenula ofunaensis(Microbiology & Fermentation Industry)
- Purification and Characterization of Xylose Isomerase of a Methanol Yeast, Candida boidinii, Which Is Involved in Sorbitol Production from Glucose(Microbilolgy & Fermentation Industry)
- α-Tocopherol Production by an Analog-resistant Strain of Euglena gracilis Z(Microbiology & Fermentation Industry)
- Involvement of Four Hormones in Thyroxine Deiodination in Several Tissues of Immature Yearling Masu Salmon, Oncorhynchus masou
- Cytochrome c Production in Jar Fermentor by a Mutant, Methylomonas sp.
- Soluble Cytochromes c of a KCN-resistant Mutant of an Obligate Methylotroph, Methylomonas sp.(Microbiology & Fermentation Industry)
- Intracisternal injection of orexin-A prevents ethanol-induced gastric mucosal damage in rats
- Enzymatic Production of D-(-)-Pantoyl Lactone from Ketopantoyl Lactone(Microbiology & Fermentation Industry)
- Crystallographic Two Sublattice System : Staggered Susceptibility and Induced Staggered Magnetization in Paramagnetic State
- Microbial Synthesis of a Tranexamic Acid Intermediate from Dinitrile
- A Newly Developed Video-Meibography System Featuring a Newly Designed Probe
- Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica(Microbiology & Fermentation Industry)
- NADH production from NAD^+ using a Formate Dehygdogenase System with Cells of a Methanol-Utilizing Bacterium
- Isolation of L-Methionine-Enriched Mutant of a Methylotrophic Yeast, Candida boidinii No.2201
- Production of Raw Cassava Starch-Digestive Glucoamylase by a 2-Deoxyglucose-Resistant Mutant of Rhizopus sp.
- 321 NADPH Production Using a Glucose Dehydrogenase System with Intact Cells and Immobilized Cells of Gluconobacter suboxydans
- Observation of Spin Echoes on Mn^ in Manganese Ferrite
- Biotin Overproduction by Biotin Analog-resistant Mutants of Bacillus sphaericus
- ELECTROMYOGRAPHIC STUDIES ON THE BACK, BUTTOCK AND ABDOMINAL MUSCLE ACTIVITIES DURING SPINAL MOVEMENTA(PART 1)