A Processing Technique for Cell Surfaces Using Gas Cluster Ions for Imaging Mass Spectrometry
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概要
- 論文の詳細を見る
We developed a processing technique for biological samples, that is, animal cells, for imaging mass spectrometry using gas cluster ion beams. Secondary ion mass spectrometry has been investigated for cellular imaging with high spatial resolution. Nanoscale processing techniques are needed for cellular level imaging, for example, for removing contamination from cell surfaces and exposing the insides of cells. In this study, we compared the mass spectrum of the unetched cell sample to that of the cell irradiated with an Ar cluster ion beam. The contamination was removed from the sample surface and the signals of intracellular components were detected. The results indicate that the cells were etched with low damage using the gas cluster ion beam. Finally, we compared the ion image of an unetched cell to that of an etched cell and demonstrated that the technique of cluster ion irradiation could be applied to the processing of samples for cellular level imaging mass spectrometry.
- 2009-06-01
著者
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青木 学聡
京都大学 大学院工学研究科 電子工学専攻
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YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
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NAKATA YOSHIHIKO
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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Matsuo Jiro
Quantum Science And Engineering Center Kyoto University
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Seki Toshio
Department Of Nuclear Engineering Kyoto University
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Seki Toshio
Department Of Cardiovascular Surgery Nara Prefectural Mimuro Hospital
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Aoki Takaaki
Department Of Electronic Science And Engineering Kyoto University
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Aoki Takaaki
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Nakata Yoshihiko
Department Of Nuclear Engineering Kyoto University
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Nakata Yoshihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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青木 学聡
クラスターイオンビーム集中研究体
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Ichiki Kazuya
Department Of Nuclear Engineering Kyoto University
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NINOMIYA Satoshi
Quantum Science and Engineering Center, Kyoto University
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Ninomiya Satoshi
Quantum Science And Engineering Center Kyoto University
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Yamada Hideaki
Department Of Nuclear Engineering Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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