Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
スポンサーリンク
概要
- 論文の詳細を見る
A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.
- 社団法人 日本農芸化学会の論文
著者
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Inagaki Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
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Yamada H
Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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TANI Yoshiki
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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IZUMI Yoshikazu
Department of Biotechnology, Tottori University
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KANO Yoshiaki
Department of Agricultural Chemistry, Kyoto University
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KAWASE Naoki
Department of Agricultural Chemistry, Kyoto University
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
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Tani Yoshiki
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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