Gene Cloning and Characterization of an Acidic Xylanase from Acidobacterium capsulatum

スポンサーリンク

概要

• 論文の詳細を見る

• The gene xynA encoding an acid endo-β-1,4-xylanase from an acidophilic bacterium, Acidobacterium capsulatum 161,was cloned and expressed in Escherichia coli. The nucleotide sequence of the 1.6-kb DNA fragment containing xynA was analyzed, revealing an open reading frame of 1,215 bp encoding a peptide of 405 amino acid residues. The deduced amino acid sequence of XynA was very similar to other xylanases that are from the glycosyl hydrolase family 10. XynA was purified to homogeneity by SDS-polyacrylamide gel electrophoresis from E. coli transformants. The molecular mass and isoelectric point of XynA were 41 kDa and 7.3,respectively. The xylanase activity of the cloned XynA is an endo-acting enzyme that shows optimal activity at pH 5.0 and 65℃, and is stable pH between 3.0 and 8.0. The K_m and V_<max> with oat spelt xylan as a substrate at pH 5.0 and 30℃ are 3.5 mg/ml and 403 μmol/min/mg.

• 社団法人日本農芸化学会の論文
• 1998-06-23

著者

• Tamura Takashi

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni

• Inagaki Kenji

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni

• Tanaka H

  Faculty Of Pharmacy Meijo University

• Furutani Y

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Inagaki Kenji

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• Tamura T

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• TANAKA HIDEHIKO

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Toyama Hirohide

  Institute For Chemical Research Kyoto University

• Tanaka Hidehiko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tanaka Haruyuki

  Chemical Branch Faculty Of Engineering And Kansai University High Technology Research Center Kansai

• Mukai K

  Hayashibara Biochemical Lab. Inc. Okayama Jpn

• Tamura Takashi

  Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo

• NAKAHIRA Ken

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• MUKAI Kazuhisa

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Mukai Kazuhisa

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Nakahira Ken

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Inagaki K

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• TANAKA Hiromi

  Department of Physics Faculty of Education, Saga University

• INAGAKI KENJI

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

関連論文

• Production improvement of antifungal, antitrypanosomal nucleoside sinefungin by rpoB mutation and optimization of resting cell system of Streptomyces incarnatus NRRL8089(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Restriction Endonuclease Aor13HI from Acidiphilium organovorum 13H, a New Isoschizomer of BspMII : Purification and Characterization
• IS075 Thrombectomy with AngioJet Catheter in Native Coronary Arteries for Patients with Acute or Recent Myocardial Infarction
• Evaluation of depth distribution function for AR-XPS using synchrotron radiation hard X-ray (Extended abstracts book of the International Workshop for Surface Analysis and Standardization '09 (iSAS-09))
• Cloning and sequencing of cDNAs encoding walleye pollack α-skeletal actin isoforms
• Amino acid sequence of phospholipase A_2 from the pyloric ceca of starfish Asterina pectinifera
• Amino Acid Sequence of Troponin-I from Akazara Scallop Striated Adductor Muscle
• Thioredoxin Reductase 1 Is Important for Selenoprotein Biosynthesis in HeLa Cells
• Cloning and Expression of the Thiobacillus Ferroxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia coli
• Growth Inhibition of Acidiphilium Species by Organic Acids Contained in Yeast Extract
• Isolation and Characterization of Restriction Endonucleases from Acidiphilium sp. 16R and 22M
• Revised Structures for Senegalensin and Euchrenone b_.
• Convenient Synthesis of Cyclohexa[a]pyrrolo[2,1-b][3]benzazepine, A Cephalotaxus Alkaloid Analogue.
• Purification and Some Characteristics of a Monomeric Alanine Racemase from an Extreme Thermophile, Thermus thermophilus
• Alanine Racemase from an Acidophile, Acidiphilium organovorum : Purification and Characterization
• IL-10 Augments Antibody Production in in Vitro Immunized Lymphocytes by Inducing a Th2-Type Response and B Cell Maturation
• Effects of the Principal Nutrients on Lovastatin Production by Monascus pilosus
• Erysubins C-F, Four Isoflavonoids from Erythrina suberosa var.glabrescences.
• Two New Isoflavones from Erythrina suberosa var.glabrescences.
• Metabolic Fate of Sarcosyl-^C-glycine in Normal Young Rats
• Metabolic Fate of β-Aspartyl-^C-glycine in Normal Young Rats
• Structural Analysis of the L-Methionine γ-Lyase Gene from Pseudomonas putida^1
• Anti-Peptide Antibody Production Elicited by in Vitro Immunization of Human Peripheral Blood Mononuclear Cells
• PJ-628 Oxidant Stress-Mediated Myocardial Cell Injury in Selenium-Deficient Mice : Age-Dependent Roles of Glutathion Peroxidase Inhibition(Cardiomyopathy, Basic and Clinical 2 (M) : PJ105)(Poster Session (Japanese))
• A new Erythrina alkaloid from Erythrina herbacea
• P-493 Studies on Constituents of Erythrina variegata (Leguminosae)
• Four New Isoflavonoids and a New 2-Arylbenzofuran from the Roots of Erythrina variegata
• Erythrinan Alkaloids and Isoflavonoids from Erythrina poeppigiana.
• Two New Isoflavonoids from Erythrina variegata.
• Two New Erythrina Alkaloids from Erythrina x bidwillii.
• Erythrina Alkaloid from Erythrina x bidwilli.
• Three Isoflavone from Erythrina orientalis.
• An Isoflavon from Erythrina x bidwillii.
• Two Pterocarpans from Erythrina Orientalis.
• Two New Erythrinan Alkaloids from Erythrina x bidwillii
• Three Pterocarpans from Erythrina crista-galli.
• A Pterocarpan from Erythrina orientalis.
• Inhibition of Sulfur Use by Sulfite Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Purificaion and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Effects of Polyamine on the Growth of Acidiphilium facilis 24R under Acidic Conditions(Microbiology & Fermentation Industry)
• The Mechanism of Copper Leaching by Intact Cells of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• The Regulation of Sulfur Use by Ferrous Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Purification and Characterization of Glycerol 3-Phosphate Dehydrogenase from Two Types of Acidiphilium sp.(Biological Chemistry)
• Effect of Co^ on the GSH Uptake and Release by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Mechanism of Tetravalent Manganese Reduction with Elemental Sulfur by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• 4-(Benzoylindolizinyl)butyric Acids ; Novel Nonsteroidal Inhibitors of Steroid 5α-Reductase. III.^
• 4-(1-Benzoylindol-3-yl)butyric Acids and FK143 : Novel Nonsteroidal Inhibitors of Steroid 5α-Reductase (II)
• (1-Benzylinodole-3-yl)alkanoic Acids : Novel Nonsteroidal Inhibitors of Steroid 5α-Reductase (I)
• Total Synthesis of WS9326A, a Potent Tachykinin Antagonist from Streptomyces violaceoniger
• Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
• Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation
• Purification and Properties of an Acidic β-Glucosidase from Acidobacterium capsulatum
• The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
• Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
• Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
• Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
• Synthesis of Four Stereoisomers of 1,4-Thiazane-3-carboxylic Acid 1-Oxide via the Asymmetric Transformation (combined isomerization-preferential crystallization) of 1,4-Thiazane-3-carboxylic Acid
• Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
• Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
• Isolation and Some Properties of Acid Phosphatase-1^1 from Tomato Leaves(Biological Chemistry)
• Crystal Structure of the Pyridoxal 5'-phosphate Dependent L-Methionine γ-Lyase from Pseudomonas putida
• Structure of the Antitumour Enzyme L-Methionine γ-Lyase from Pseudomonas putida at 1.8A Resolution
• Preparation and Characterization of Insulin-Loaded Acrylic Hydrogels Containing Absorption Enhancers
• Quantitative Evaluation of the Bitterness of Commercial Medicines Using a Taste Sensor
• Role of Tyrosine 114 of L-Methionine γ-lyase from Pseudomonas putida
• Conversion of α-Keto Acids to D-Amino Acids by Coupling of Four Enzyme Reactions
• Relationship between production of hydroxyl radicals and degradation of wood, crystalline cellulose, and a lignin-related compound or accumulation of oxalic acid in cultures of brown-rot fungi
• Solid-Phase Tandem Radical Addition-Cyclization Reaction : Triethylborane-Induced Reaction of Oxime Ethers Anchored to Polymer Support
• Physical and Chemical Properties of an Extracellular Low-Molecular-Weight Substance from the Brown-Rot Basidiomycete Fomitopsis palustris
• Stannyl Radical Addition-Cyclization of Oxime Ethers Connected with Olefins
• Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6
• Role and Behavior of Benthic Microbes Able to Degrade Herbicide Atrazine in Naturally Derived Water/sediment Microcosm
• Bilirubin Dehydrogenase, an Enzyme in Aspergillus ochraceus IB-3 Useful for Diagnostic Measurement of Bilirubin(Microbiology & Fermentation Technology)
• Evaluation of Myopathy Risk for HMG-CoA Reductase Inhibitors by Urethane Infusion Method
• Purification and Characterization of an Acid Trehalase from Acidobacterium capsulatum
• State of Tryptophyl Residues and Stability of Recombinant Human Matrix metalloproteinase 7 (Matrilysin) as Examined by Fluorescence^1
• A Novel Class of Highly Potent and Orally Active Nonpeptide Bradykinin B_2 Receptor Antagonists
• Immunogold labeling of an extracellular substance producing hydroxyl radicals in wood degraded by brown-rot fungus Tyromyces palustris
• Anti-Oxidative Compounds in Barley Tea
• 9-Oxo-neoprocurcumenol from Curcuma aromatica (Zingiberaceae) as an Attachment Inhibitor against the Blue Mussel, Mytilus edulis galloprovincialis(Organic Chemistry)
• Separation of Chromosomal DNA Molecules of Candida maltosa on Agarose Gels Using the OFAGE Technique(Microbiology & Fermentation Industry)
• Synthesis of Optically Active 1,4-Thiazane-3-carboxylic Acid via Optical Resolution by Preferential Crystallization of (RS)-2-Amino-3-[(2-chloroethyl) sulfanyl] propanoic Acid Hydrochloride
• Synthesis and Fungicidal Activities of Silicon-Containing Derivatives of 2-Aryl-3-(1H-1, 2, 4, -triazol-1-yl)propanenitriles
• Synthesis of 1-Triazolyl-4-trimethylsilyl-2-butanol and 1-Triazolyl-5-trimethylsilyl-2-pentanol Derivatives and an Investigation of Their Fungicidal Activities
• Synthesis and Systemic Fungicidal Activity of Silicon-Containing Azole Derivatives
• Physicochemical Properties of Glyme-Li Salt Complexes as a New Family of Room-temperature Ionic Liquids
• 476 Isolation and Characterisation of Alanine Racemase from Extreme Thermophile, Thermus thermophilus
• Synthesis of Chiral Calix[4]resorcinarenes via Mono-O-benzylation. Complexation Behavior with a Chiral Trimethylammonium Compound
• Selenite Assimilation into Formate Dehydrogenase H Depends on Thioredoxin Reductase in Escherichia coli
• Identification and Purification of an L-Alanine Dehydrogenase Homolog that Catalyzes the Formation of L-Ornithine Lactam from Sinefungin-Producing Streptomyces incarnatus NRRL8089
• Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus ferrooxidans
• Enhanced Production of the Fluorinated Nucleoside Antibiotic Nucleocidin by a rif^R-Resistant Mutant of Streptomyces calvus IFO13200
• Characterization of Monofunctional Catalase KatA from Radioresistant Bacterium Deinococcus radiodurans(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Studies on 5-Lipoxygenase Inhibitors. II. Discovery, Optical Resolution and Enantioselective Synthesis of FR110302,a Highly Potent Non-redox Type-5-Lipoxygenase Inhibitor
• Studies on 5-Lipoxygenase Inhibitors. I. Synthesis and 5-Lipoxygenase-Inhibitory Activity of Novel Hydroxamic Acid Derivatives
• Gene Cloning and Characterization of an Acidic Xylanase from Acidobacterium capsulatum
• Laccase Production and One-electron Oxidation Activity in Wood Degradation by the Soft-rot Deuteromycete Graphium sp.
• PJ-499 Lateral E/Ea is the Most Reliable Predictor of Left Ventricular Filling Pressure in Patients with Old Myocardial Infarction(Echo/Doppler(16)(I),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• Anti-Peptide Antibody Production Elicited by in Vitro Immunization of Human Peripheral Blood Mononuclear Cells
• Regulatory Region of Expression of Thiobacillus ferrooxidans leuB Gene in Escherichia coli

もっと見る 閉じる

スポンサーリンク