The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION

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概要

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• To characterize the molecular structure of the S alleles in Lycopersicon peruvianum, we isolated two genomic fragments that corresponded to CDNAS for S_<11> and S_<12> RNases from a genomic library of a plant with the S_<11>S_<12> genotype. The coding regions and the flanking regions in both fragments were sequenced. A single short intron was found in each gene for RNase, and it was located in the previously identified HV2 region [Chung et al. (1994) Plant Mol. Biol. 26: 757]. The site of initiation of transcription of the gene for S_<11> RNase was determined by a primer extension analysis and a putative TATA box was identified. Three short sequences that were highly homologous in the two S alleles were found within 360 bp upstream of the ATG initiation codon. It is suggested that these sequences might be involved in the regulation of transcription required for the appropriate-level or tissue-specific expression of the S genes in L. peruvianum.

• 日本植物生理学会の論文

著者

• LEE Sang

  Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center

• TANAKA Hiroshi

  Department of Pediatrics, Hirosaki University School of Medicine

• ITO TORU

  Department of Internal Medicine, Tohoku University School of Medicine

• Chung Ii-kyung

  Department Of Life Science Pohang University Of Science And Technology

• Takagi M

  Department Of Biotechnology The University Of Tokyo

• Takagi Masamichi

  Department of Agricultural Chemistry, The University of Tokyo

• NAM Hong

  Division of Molecular Life Science, Pohang University of Science and Technology

• Tanaka H

  Faculty Of Pharmacy Meijo University

• Ito T

  Univ. Tokyo Tokyo

• Lee Sang

  Department Of Advanced Materials Science And Engineering Mokpo National University

• Tanaka H

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Takahashi Maki

  Department Of Biotechnology The University Of Tokyo

• Nam Hong

  Department of Life Science and School of Environmental Engineering, Pohang University of Science and

• CHUNG Il-Kyung

  Department of Life Science, Pohang University of Science and Technology

• Toyama Hirohide

  Institute For Chemical Research Kyoto University

• Tanaka Haruyuki

  Chemical Branch Faculty Of Engineering And Kansai University High Technology Research Center Kansai

• Takagi Masamichi

  Department Of Biotechnology University Of Tokyo

• Takagi Masamichi

  Department Of Biotechnology The University Of Tokyo

• Takagi Masamichi

  Department Of Agricultural Chemistry The University Of Tokyo

• Ito Toru

  Department Of Biotechnology The University Of Tokyo

• Takagi M

  Department Of Biotechnology University Of Tokyo

• Nam H

  Divison Of Molecular Life Sciences Pohang University Of Science And Technology

• Nam Hong

  Department Of Life Science And School Of Environmental Engineering Pohang University Of Science And

• Tanaka Hiroshi

  Department Of Cardiology Jichi Medical School

• Lee S

  Division Of Applied Life Science (bk21) Environmental Biotechnology National Core Research Center An

• Takahi Masamichi

  Department Of Biotechnology The University Of Tokyo

• Tanaka Hiroshi

  Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hoshi University

• LEE Sang

  Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University

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