Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus ferrooxidans

スポンサーリンク

概要

• 論文の詳細を見る

• We constructed an overexpression system in Escherichia coli of the leuB gene coding for 3-isopropylmalate dehydrogenase in Thiobacillus ferrooxidans. E.coli harboring the plasmid we constructed, pKK leuB1, produced 17-fold the enzyme protein of the expression system previously used for purification.The substrate specificity of the enzyme was analyzed with synthetic(2R, 3S)-3-alkylmalates. The 3-isopropylmalate dehydrogenase of Thiobacillus ferrooxidans had broad specificity toward the alkylmalates.

• 社団法人日本農芸化学会の論文
• 1998-02-23

著者

• Inagaki Kenji

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni

• KAWAGUCHI Hiroshi

  Department of Orthopaedic Surgery, The University of Tokyo

• EGUCHI Tadashi

  Department of Chemistry and Materials Science, Tokyo Institute of Technology

• KAKINUMA Katsumi

  Department of Chemistry, Tokyo Institute of Technology

• Furutani Y

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Inagaki Kenji

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• Eguchi Tadashi

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Eguchi Tadashi

  Department Of Chemistry Tokyo Institute Of Technology

• TANAKA HIDEHIKO

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kakinuma Katsumi

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Tanaka H

  Institute Of Information Security

• Kawahara Hidehisa

  Department of Biotechnology, Faculty of Engineering, Kansai University

• MATSUNAMI HIDEYUKI

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Tanaka Hidehiko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tanaka Hidehiko

  Institute Of Information Security

• Hidehisa Kawahara

  Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center

• Kawaguchi Hiroshi

  Department Of Chemistry Faculty Of Science Kochi University

• Kawaguchi H

  Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo

• Matsunami Hideyuki

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Eguchi Tadashi

  Department Of Chemistry & Materials Science Tokyo Institute Of Technology

• Inagaki K

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Kawaguchi Hiroshi

  Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University

• Kawaguchi Hiroshi

  Department Of Orthopaedic Surgery The University Of Tokyo

• Kakinuma Katsumi

  Department of Chemistry & Materials Science, and Department of Chemistry, Tokyo Institute of Technology

• INAGAKI KENJI

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

関連論文

• Production improvement of antifungal, antitrypanosomal nucleoside sinefungin by rpoB mutation and optimization of resting cell system of Streptomyces incarnatus NRRL8089(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Roles of a 20 kDa Protein Associated with a Carbocycle-Forming Enzyme Involved in Aminoglycoside Biosynthesis in Primary and Secondary Metabolism
• Identification of sequence polymorphisms in two sulfation-related genes, PAPSS2 and SLC26A2, and an association analysis with knee osteoarthritis
• Isolation and Structure Elucidation of Vicenistatin M, and Importance of the Vicenisamine Aminosugar for Exerting Cytotoxicity of Vicenistatin
• Structure of NG-061, a Novel Potentiator of Nerve Growth Factor (NGF) Isolated from Penicillium minioluteum F-4627
• Cross-Layer Design for Low-Power Wireless Sensor Node Using Wave Clock
• Transient pancytopenia associated with parvovirus infection in a healthy child
• Identification of sequence polymorphisms of the COMP (cartilage oligomeric matrix protein) gene and association study in osteoarthrosis of the knee and hip joints
• Magnetic Chromatography of Magnetic Fine Particles Suspended in a Liquid with a Steel-Bead Column under a Periodically Intermittent Magnetic Field
• Laser Ablation/Inductively Coupled Plasma Mass Spectrometry with Aerosol Density Normalization
• Optimization of Operating Conditions in Individual Airborne Particle Analysis by Inductively Coupled Plasma Mass Spectrometry
• Effect of Hydrogen Addition to the Discharge Gas in Radio-Frequency Argon Glow-Discharge Mass Spectrometry for Nonconducting Samples
• Effects of Helium Addition to Radio-Frequency Argon Glow-Discharge Mass Spectrometry
• Effect of Orifice Diameter of Torch Injector Tube in Inductively Coupled Plasma Mass Spectrometry
• OJ-534 Prognostic Value of Heart Rate Variability for All Cause and Sudden Death in Patients with Renal Failure on Hemodialysis(Kidney/Renal Circulation 3 (H) : OJ65)(Oral Presentation (Japanese))
• Purification and Some Characteristics of a Monomeric Alanine Racemase from an Extreme Thermophile, Thermus thermophilus
• Alanine Racemase from an Acidophile, Acidiphilium organovorum : Purification and Characterization
• Design and Characterization of a Helium-Discharge Ionization Detector for Gas Chromatography
• Cloning of the Pactamycin Biosynthetic Gene Cluster and Characterization of a Crucial Glycosyltransferase Prior to a Unique Cyclopentane Ring Formation
• Biosynthesis of 2-Deoxystreptamine-containing Antibiotics in Streptoalloteichus hindustanus JCM 3268 : Characterization of 2-Deoxy-scyllo-inosose Synthase
• Biosynthesis of 2-Deoxystreptamine by Three Crucial Enzymes in Streptomyces fradiae NBRC 12773
• Extended Sequence and Functional Analysis of the Butirosin Biosynthetic Gene Cluster in Bacillus circulans SANK 72073
• Reaction Stereochemistry of 2-Deoxy-scyllo-inosose Synthase, the Key Enzyme in the Biosynthesis of 2-Deoxystreptamine
• First Identification of Streptomyces Genes Involved in the Biosynthesis of 2-Deoxystreptamine-containing Aminoglycoside Antibiotics : Genetic and Evolutionary Analysis of L-Glutamine : 2-deoxy-scyllo-inosose Aminotransferase Genes
• Identification of L-Glutamine : 2-Deoxy-scyllo-inosose Aminotransferase Required for the Biosynthesis of Butirosin in Bacillus circulans
• Novel Substrate Specificity of Designer 3-Isopropylmalate Dehydrogenase Derived from Thermus thermophilus HB8(Biochemistry & Molecular Biology)
• Butirosin-biosynthetic Gene Cluster from Bacillus circulans
• Effects of the Principal Nutrients on Lovastatin Production by Monascus pilosus
• Stereoselective Formation and Characterization of S-Bridged Dinuclear Λ- and Δ-[Co{Co(didentate-N,S)_3}(_D-pen-N,O,S)]-Type Complexes (didentate=2-Aminoethanethiolate, _L-Cysteinate, and _D-Penicillaminate (_D-pen))
• Photochemistry of Cobalt(III) Complexes. II. Cobalt(III) Complex with a Co-C-S Three-Membered Ring Prepared by Photodecarboxylation of 2,2'-Thiodiacetato Complex. Crystal Structure of (-)^_-fac-trans(NH,O)-[Co(CH_2SCH_2COO)(dien)]ClO_4
• Structural Analysis of the L-Methionine γ-Lyase Gene from Pseudomonas putida^1
• Antineutrophil cytoplasmic antibody-positive crescentic glomerulonephritis associated with propylthiouracil therapy
• Cross-Layer Design for Low-Power Wireless Sensor Node Using Wave Clock
• Inhibition of Sulfur Use by Sulfite Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Purificaion and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
• Effects of Polyamine on the Growth of Acidiphilium facilis 24R under Acidic Conditions(Microbiology & Fermentation Industry)
• Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
• Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
• Crystal Structure of the Pyridoxal 5'-phosphate Dependent L-Methionine γ-Lyase from Pseudomonas putida
• Structure of the Antitumour Enzyme L-Methionine γ-Lyase from Pseudomonas putida at 1.8A Resolution
• A Quantitative Analysis of Rat Osteoporosis Model with a Microfocus X-ray Tube and Digital Radiography System
• Low Expression of Adhesion Molecules in a Case of Cutaneous T-cell Lymphoma
• Neurologic Level Diagnosis of Cervical Stenotic Myelopathy
• A 58-μW Single-Chip Sensor Node Processor with Communication Centric Design
• Role of Tyrosine 114 of L-Methionine γ-lyase from Pseudomonas putida
• Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6
• Purification and Characterization of an Acid Trehalase from Acidobacterium capsulatum
• A 50% Power Reduction in H.264/AVC HDTV Video Decoder LSI by Dynamic Voltage Scaling in Elastic Pipeline(VLSI Architecture,VLSI Design and CAD Algorithms)
• A Case of Polypoid Clear Cell Acanthoma on the Scrotum
• Adult Onset of Inflammatory Linear Verrucous Epidermal Nevus
• A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling
• Vertebral hemangioma compressing the thoracic spinal cord : application of computer-aided navigation and intraoperative spinal sonography for surgery through anterior and posterior approaches
• Revision cervical spine surgery using transarticular or pedicle screws under a computer-assisted image-guidance system
• Can Laminoplasty Maintain the Cervical Alignment Even When the C2 Lamina Is Contained?
• Postoperative Expansion of Intramedullary High-Intensity Areas on T2-Weighted Magnetic Resonance Imaging After Cervical Laminoplasty
• Retroodontoid pseudotumor resected by a high cervical lateral approach in a rheumatoid arthritis patient : a case report
• Cervical myelopathy in an adult due to atlantoaxial subluxation associated with Down syndrome : a case study
• Image-guided surgery for cervical disorders in rheumatoid arthritis
• Association of Bone Metabolism Regulatory Factor Gene Polymorphisms With Susceptibility to Ossification of the Posterior Longitudinal Ligament of the Spine and Its Severity
• Computer-assisted anterior spinal surgery for a case of recurrent giant cell tumor
• Long-Term Results of Operative Treatment for Cervical Spondylotic Myelopathy in Patients With Athetoid Cerebral Palsy : An Over 10-Year Follow-Up Study
• Long-Term Results of Double-Door Laminoplasty for Cervical Stenotic Myelopathy
• Determination of Ultratrace Indium in Water Sample by Electrothermal Atomic Absorption Spectrometry after Preconcentration with Solvent Extraction and Back Extraction
• Determination of Ultratrace Gallium in River Water by Electrothermal Atomic Absorption Spectrometry after Preconcentration with Solvent Extraction and Back Extraction
• Diet and lifestyle associated with increased bone mineral density : cross-sectional study of Japanese elderly women at an osteoporosis outpatient clinic
• Risk factors for knee osteoarthritis in Japanese men : a case-control study
• Selenite Assimilation into Formate Dehydrogenase H Depends on Thioredoxin Reductase in Escherichia coli
• Identification and Purification of an L-Alanine Dehydrogenase Homolog that Catalyzes the Formation of L-Ornithine Lactam from Sinefungin-Producing Streptomyces incarnatus NRRL8089
• Versatile Synthon for Chirally β-Deuterated L-Amino Acids and Synthesis of (3R)- and (3S)-[3-^2H_1]-L-Serine
• Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus ferrooxidans
• Biosynthesis of (2R)-4-Amino-2-hydroxybutyric Acid, Unique and Biologically Significant Substituent in Butirosins
• Enhanced Production of the Fluorinated Nucleoside Antibiotic Nucleocidin by a rif^R-Resistant Mutant of Streptomyces calvus IFO13200
• 3-Isopropylidenemalic Acid:A Mechanism-based Inhibitor of 3-Isopropylmalate Dehydrogenase
• Coenzyme Activity of NAD Analogs for 3-Isopropylmalate Dehydrogenase from Thermus thermophilus HB8
• The Incomplete Cubane Complexes Having Co_3O_4 or Mo_3S_4 Core with the N-N-O Type Tridentates; L-histidinato and Ethylenediamine-N-acetato
• Mutation in Aging Suppressor Gene, Klotho, Causes Decreases in Bone Formation and Resorption in Mice(Advances in Connective Tissue Research in Bone and Joint)
• Synthesis of 2-O-Methyl Ether and 1-Carboxamide Derivatives of (2R,3S)-3-Isopropylmalic Acid and Their Interaction with Thermophilic 3-Isopropylmalate Dehydrogenase
• Synthesis of NG-061 and Its Analogs, and Their Biological Evaluation as an Enhancer of Nerve Growth Factor
• Isolation and Characterization of a New Pyrano[4', 3': 6, 7]naphtho[1, 2-b]xanthene Antibiotic FD-594
• Optical Isolator for Orthogonally Polarized Two-Frequency Laser
• Characterization of Monofunctional Catalase KatA from Radioresistant Bacterium Deinococcus radiodurans(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Gene Cloning and Characterization of an Acidic Xylanase from Acidobacterium capsulatum
• Significance of the 71-Membered Macrocyclic Structure Found in Archaeal Membrane Lipids : Model Studies of the Macrocyclic Tetraether Diphospholipids by Calorimetric, ^PNMR, and Electron Microscopic Analyses
• A 0.3-V operating, Vth-variation-tolerant SRAM under DVS environment for memory-rich SoC in 90-nm technology era and beyond
• Exposure of macrophage-like cells to titanium particles does not affect bone resorption, but inhibits bone formation
• Biosynthesis of Archaeal Membrane Lipids : Digeranylgeranylglycerophospholipid Reductase of the Thermoacidophilic Archaeon Thermoplasma acidophilum
• Curative Effect of Combined Treatment With Alendronate and 1α-Hydroxyvitamin D_3 on Bone Loss by Ovariectomy in Aged Rats
• Synthesis and X-Ray Structural Study of Magnesium (1, 3-Propanediaminetetraacetato)nickelate(II) Octahydrate, Mg[Ni(1, 3-pdta)]・8H_2O. Structural Parameters and Strain Analysis of [M(1, 3-pdta)]^ Complexes in Relation to Their Octahedral Distortion
• Preparation and Crystal Structure of the trans(O^5) Isomer of Magnesium(ethylenediamine-N,N,N'-triacetato-N'-3-propionato)cuprate(II)Octahydrate, Mg[Cu(ed3ap)]・8H_2O. Stereochemistry of Octahedral Cu(II)-EDTA-Type Complexes in Relation to the Structure of
• Synthesis and Characterization of Hexadentate Cobalt(III) Complexes with New edta-Type Ligands Part 5. Structure and Absolute Configuration of the trans(O_5)(-)_-Isomer of Lithium (Ethylenediamine-N, N, N'-triacetato-N'-3-propionato)cobaltate(III)Mon
• A polymorphic variant at the Werner helicase(WRN)gene is associated with bone density, but not spondylosis, in postmenopausal women
• Prevalence of knee osteoarthritis, lumbar spondylosis, and osteoporosis in Japanese men and women : the research on osteoarthritis/osteoporosis against disability study
• Satisfaction and the Correlative Outcomes in Cervical Laminoplasty
• Association of low dietary vitamin K intake with radiographic knee osteoarthritis in the Japanese elderly population : dietary survey in a population-based cohort of the ROAD study
• Determination of Ultratrace Beryllium in Natural Water by Electrothermal Atomic Absorption Spectrometry after Preconcentration with One-Drop Solvent
• Regulatory Region of Expression of Thiobacillus ferrooxidans leuB Gene in Escherichia coli
• Impact of knee and low back pain on health-related quality of life in Japanese women : the Research on Osteoarthritis Against Disability (ROAD)
• Stereoselective Formation of[Cr(edda or eddp)(N-N)-Type Complexes with 1, 10-Phenanthroline(edda=ethylenediamine-N, N'-diacetate;eddp=ethylenediamine-N, N'-dipropionate)
• Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system
• Normal and threshold values of radiographic parameters for knee osteoarthritis using a computer-assisted measuring system (KOACAD) : the ROAD study

もっと見る 閉じる

スポンサーリンク