Characterization of Monofunctional Catalase KatA from Radioresistant Bacterium Deinococcus radiodurans(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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Catalase plays a key role in protecting cells against toxic reactive oxygen species. Here we report on the cloning, purification and characterization of a catalase (KatA, DR1998) from the extremely radioresistant bacterium Deinococcus radiodurans. The size of purified D. radiodurans KatA monomer was 65 kDa while gel filtration revealed that the size of the enzyme was 240 kDa, suggesting that KatA formed a homotetramer in solution. Purified KatA displayed a final specific activity of 68,800U/mg of protein. The catalase activity of KatA was inhibited by sodium azide, sodium cyanide and 3-amino-1, 2, 4-triazole. The absorption spectrum of KatA exhibited a Soret band at 408nm. The position of the spectral peak remained unchanged following reduction of KatA with dithionite. No peroxidase activity was found for KatA. These results demonstrate that D. radiodurans KatA is a typical monofunctional heme-containing catalase. The stability of KatA with respect to H_2O_2 stress was superior to that of commercially available Aspergillus niger and bovine liver catalases. The relative abundance of KatA in cells in addition to the H_2O_2 resistance property may play a role in the survival strategy of D. radiodurans against oxidative damage.
- 社団法人日本生物工学会の論文
- 2006-04-25
著者
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Tamura Takashi
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Inagaki Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Narumi Issay
Gene Resource Research Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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UMEDA Koichi
Amano Pharmaceutical Co., Ltd.
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Umeda Koichi
Amano Enzyme Inc. Gifu R&d Center
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Tamura Takashi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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Kobayashi Issei
Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama Un
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Sghaier Haitham
Gene Resource Research Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency
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Yamaguchi Shotaro
Amano Enzyme Inc., Gifu R&D Center
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Kobayashi Issei
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Sghaier Haitham
Gene Resource Research Group Quantum Beam Science Directorate Japan Atomic Energy Agency:department
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Yamaguchi Shotaro
Amano Enzyme Inc. Gifu R&d Center
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Tamura Takashi
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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