Purification and Characterization of an Acid Trehalase from Acidobacterium capsulatum

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概要

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• We purified an acid trehalase (EC 3.2.1.28, α, α'-trehalose glucohydrolase) from an acidophilic bacterium, Acidobacterium capsulatum. The enzyme was homogeneous based on polyacrylamide gel electrophoresis, and was composed of a single polypeptide chain with a molecular mass of 57 kDa. Maximum trehalase activity was observed at pH 2.5. The acid trehalase exhibited an apparent K_m of 1.0 mM for trehalose at 30℃ and pH 3.0. The trehalase was located in the periplasmic space. The activity of the enzyme was activated by 1.0 mM MnCl_2 or CoCl_2, and inhibited by 1.0 mM PbCl_2, HgCl_2, NiCl_2, p-chloromercuribenzoate, N-ethylmaleimide, monoiodoacetate, or EDTA. The enzyme showed high specificity for trehalose. It was found that an equimolar mixture of α-D-glucose and β-D-glucose was formed on hydrolysis of trehalose by the trehalase.

• 社団法人日本生物工学会の論文
• 2001-02-25

著者

• Tamura Takashi

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni

• Inagaki Kenji

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni

• Tanaka H

  Faculty Of Pharmacy Meijo University

• Furutani Y

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Inagaki Kenji

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• Tamura T

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• TANAKA HIDEHIKO

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Tanaka H

  Department Of Agricultural Chemistry Faculty Of Agriculture Utsunomiya University

• Tanaka H

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tanaka H

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd.

• Toyama Hirohide

  Institute For Chemical Research Kyoto University

• Tanaka Hidehiko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tanaka Haruyuki

  Chemical Branch Faculty Of Engineering And Kansai University High Technology Research Center Kansai

• UENO NAOYA

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Tanaka Hiroyoki

  Faculty Of Pharmaceutical Sciences Kyushu University

• Tamura Takashi

  Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo

• Inagaki Kenji

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Ueno Naoya

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Inagaki K

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• TANAKA Hiromi

  Department of Physics Faculty of Education, Saga University

• INAGAKI KENJI

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

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