Identification of cDNA Clones Coding for the Style Specific S_<11a>-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
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概要
- 論文の詳細を見る
We isolated 18 cDNA candidates that were expected to encode parts of the self-incompatibility-associated RNase of Lycopersicon peruvianum PI 126441 (S_<11a>-plant) from a style cDNA library. Polymerase chain reaction was used with oligonucleotides derived from conserved amino acid sequences of RNases from fungi and S-associated RNases in other species of Solanaceae. Two of these clones (both do not have full length sequences) gave 826-bp and 730-bp sequences, and they had the same open reading frame, named ORF-1. Comparison of the deduced amino acid sequence of ORF-1 with S-associated RNase of other Solanaceous plants showed a high degree of similarity. mRNA encoding this ORF-1 was about 1000 bases long and detected only in the style tissue by Northern analysis. Using one of the cDNA clones as a probe, we detected sequence variability among three different S-genotypes of randomly chosen wild-type tomatoes by Southern analysis. From these results, it was concluded that ORF-1 encodes the self-incompatibility associated S-glycoprotein of L. peruvianum PI 126441 (S_<11a>-plant).
- 社団法人日本農芸化学会の論文
- 1993-07-23
著者
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ITO TORU
Department of Internal Medicine, Tohoku University School of Medicine
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Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Chung Ii-kyung
Department Of Life Science Pohang University Of Science And Technology
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Tanaka H
Faculty Of Pharmacy Meijo University
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Ito T
Univ. Tokyo Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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Nakata Kengo
Department of Agricultural Chemistry, The University of Tokyo
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Tanaka Hiroshi
Kagome Research Institute
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Nakata K
Japan Bioindustry Assoc. Tokyo Jpn
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Nakata Kengo
Research Institute Kagome Company
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Ito Toru
Department Of Biotechnology The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Ogura A
Meiji University
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