Production of Recombinant Der fI (a major mite allergen) by Aspergillus oryzae

概要

論文の詳細を見る
Der fI is a major mite allergen. To produce Der fI by Aspergillus oryzae, we placed a DNA fragment encoding precursor-type recombinant Der fI E (-1) K (reDer fI E (-1) K), which had the C-terminal amino acid of the pro-se-quence (Glu) changed to Lys, downstream of the glaA gene promoter and introduced it into Aspergillus oryzae. In liquid culture, most of the reDer fI E (-1) K produced by the transformants was degraded when culture was shaken vigorously. However, the degradation of reDer fI E (-1) K was suppressed when it was shaken gently. The processed reDer fI E (-1) K could be obtained after lysylendopeptidase and endoglycosidase Hf (Endo Hf) treatment. The yield of processed reDer fI E (-1) K was 8 mg/l. When the transformant was grown on a wheat bran culture, the yield of processed reDer fI E (-1) K reached 48mg/kg. Because processed reDer fI E (-1) Ks obtained from both cultures had almost the same IgE-binding activity and elicited the same skin reaction as native Der fI, they could be very useful for diagnostic purposes or immunotherapy.
社団法人日本農芸化学会の論文
1999-04-23