Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
スポンサーリンク
概要
- 論文の詳細を見る
Two chitin synthase genes, designated chsA and chsB, were isolated from Aspergillus nidulans with the Saccharomyces cerevisiae CHS2 gene as the hybridization probe. Nucleotide sequencing showed that chsA and chsB encoded polypeptides consisting of 1013 and 916 amino acid residues, respectively ; the hydropathy profiles of the enzymes were similar to those of other fungal chitin synthases. Northern analysis indicated that both genes were transcribed, suggesting that cellular chitin in A. nidulans is synthesized by at least two chitin synthases. For examination of the roles of the chitin synthase genes in cell growth, gene disruption experiments were done. The chsA disruptant grew as well as the wild-type strain, but the chsB disruptant had severe growth defects that could not be overcome by the addition of 1.2M sorbitol as an osmotic stabilizer. These findings suggested that chsB but not chsA is essential for hyphal growth.
- 社団法人日本農芸化学会の論文
- 1994-10-23
著者
-
Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
-
Takagi M
Department Of Biotechnology The University Of Tokyo
-
Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
-
Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
-
Takaya N
Univ. Tsukuba Ibaraki Jpn
-
Takaya Naoki
Department Of Biotechnology The University Of Tokyo:(present Address)institute Of Applied Biochemist
-
Ohta A
Dep. Of Biotechnology The Univ. Of Tokyo
-
Ohta A
Univ. Tokyo Tokyo
-
Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
-
KOJIMA Nobuko
Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University
-
HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
-
Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
-
Takahashi Maki
Department Of Biotechnology The University Of Tokyo
-
YANAI Koji
Department of Biotechnology, The University of Tokyo
-
Takagi Masamichi
Department Of Biotechnology University Of Tokyo
-
Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
-
Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
-
Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
-
Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
-
Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
-
Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
-
Takagi M
Department Of Biotechnology University Of Tokyo
-
Yanai Koji
Department Of Biotechnology The University Of Tokyo:bio Science Laboratories Meiji Seika Kaisha Ltd.
-
Kojima Nobuko
Department Of Agricultural Chemistry The University Of Tokyo
-
Ogura A
Meiji University
-
Takahi Masamichi
Department Of Biotechnology The University Of Tokyo
-
YANAI Koji
Department of Agricultural Chemistry, The University of Tokyo
関連論文
- What is the best index of glycemic control in patients with diabetes mellitus on hemodialysis?
- Evidence That the Expression of the Gene for NADPH-Cytochrome P-450 Reductase is n-Alkane-inducible in Candida maltosa
- Evolutionary Position of n-Alkane-assimilating Yeast Candida maltosa Shown by Nucleotide Sequence of Small-subunit Ribosomal RNA Gene
- Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
- Isolation and Characterization of ECT1 Gene Gncoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae^1
- Significance of Atrial and Brain Natriuretic Peptides in Patients with Diabetes Mellitus Undergoing Hemodialysis
- Involvement of a Glu71-Arg64 Couple in the Access Channel for NADH in Cytochrome P450nor
- Nitrous Oxide-forming Codenitrification Catalyzed by Cytochrome P450nor
- 女性のライフサイクルと骨塩量(BMD)に影響を及ぼす多様な因子
- 当科におけるホルモン補充療法の現状
- Changes of Bone Mineral Density in Pregnant and Postpartum Women
- Expression of Calbindin-D9k, VDR and Cdx-2 Messenger RNA in the Process by Which Fructooligosaccharides Increase Calcium Absorption in Rats
- The Effect of Fructooligosaccharides was Analyzed by cDNA Expression Arrays
- Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
- Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
- Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in Saccharomyces cerevisiae
- Transcriptional Control of Nitric Oxide Reductase Gene (CYP55) in the Fungal Denitrifier Fusarium oxysporum(Microbiology & Fermentation Technology)
- Nitrate Reductase-Formate Dehydrogenase Couple Involved in the Fungal Denitrification by Fuysarium oxysporum
- A Large-Capacity Service Control Node Architecture Using Multicasting Access to Decentralized Databases in the Advanced Intelligent Network(IEICE/IEEE Joint Special Issue on Autonomous Decentralized Systems and Systems' Assurance)
- Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
- Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
- Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
- Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
- Nonparasitic solitary huge liver cysts causing intracystic hemorrhage or obstructive jaundice
- Xanthogranulomatous Cholecystitis with a Liver Abscess and Metastatic Endophthalmitis : Report of a Case
- Production of Recombinant Der fI (a major mite allergen) by Aspergillus oryzae
- Production of Recombinant Der fI with the Native IgE-Binding Activity Using a Baculovirus Expression System
- Involvement of LEM3/ROS3 in the Uptake of Phosphatidylcholine with Short Acyl Chains in Saccharomyces cerevisiae
- Pseudomonas fluorescens KKL101, a Benzoic Acid Degrader in a Mixed Culture That Degrades Biphenyl and Polychlorinated Biphenyls
- The Wound-Healing Effect of Fibrin Glue for Tracheal Anastomosis in Experimental Pulmonary Surgery
- Reinforcing Effect of the New Synthetic Bioabsorbable Sealant (ADVASEAL^) for Bronchial Stumps after Pulmonary Resections in Pigs
- Construction of an Insertion Vector for Gene Targeting of Chicken Lens-specific Gene
- Construction of a Genetic Transformation System for a Freeze-tolerant Yeast Kluyveromyces thermotolerans
- Isolation and Sequencing of a Gene, C-ADE1, and Its Use for a Host-Vector System in Candida maltosa with Two Genetic Markers(Microbiology & Fermentation Industry)
- Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
- Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
- Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
- Isolation of Flavohemoglobin from the Actinomycete Streptomyces antibioticus Grown without External Nitric Oxide Stress
- Unusual Transcription Regulation of the niaD Gene under Anaerobic Conditions Supporting Fungal Ammonia Fermentation
- Denitrification of Nitrate by the Fungus Cylindrocarpon tonkinense(Biochemistry & Molecular Biology)
- A Possible Role of NADPH-Dependent Cytochrome P450nor Isozyme in Glycolysis under Denitrifying Conditions(Biochemistry & Molecular Biology)
- Formate-forming Fungal Catabolic Pathway to Supply Electrons to Nitrate Respiration(Biochemistry & Molecular Biology)
- Hybrid Respiration in the Denitrifying Mitochondria of Fusarium oxysporum
- Expression of Plant Group 2 and Group 3 lea Genes in Saccharomyces cerevisiae Revealed Functional Divergence among LEA Proteins
- Video-Assisted Thoracic Surgery for Patients with Lung Cancer and Interstitial Pneumonia
- Preoperative Evaluation of a Tracheal Bronchus by Three-Dimensional 64-Row Multidetector-Row Computed Tomography (MDCT) Bronchography and Angiography : Report of a Case
- The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
- Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
- Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
- Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
- Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
- Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
- Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
- Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
- Transformation of a Leu^- Mutant of Rhizopus niveus with the leuA Gene of Mucor circinelloides
- Preparation of Protoplasts of Rhizopus niveus and Their Transformation with Plasmid DNA(Microbiology & Fermentation Industry)
- Isolation and Some Properties of Acid Phosphatase-1^1 from Tomato Leaves(Biological Chemistry)
- High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Isolation of a Genomic Clone Encoding RNase Rh of Rhizopus niveus and Characterization of Its 5-Terminal Region
- Pure Dehydro-L-ascorbic Acid Prepared by O2-Oxidation of L-Ascorbic Acid with Active Charcoal as Catalyst
- Quinoxalines Derived from D-Xylose and o-Phenylenediamine under Acidic Refluxed Conditions
- Low Dose Iron Supplementation with Fructooligosaccharides Improves Iron Status and Aerobic Capacity in Women under Moderate Physical Training
- Effect of Dietary Short-Chain Fructooligosaccharides on the Cecal Microflora in Gastrectomized Rats
- Dissimilatory Nitrate Reduction Metabolisms and Their Control in Fungi(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Separation of Chromosomal DNA Molecules of Candida maltosa on Agarose Gels Using the OFAGE Technique(Microbiology & Fermentation Industry)
- Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
- The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
- Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Misoflded Membrane-Bound Cytochrome P450 Activates KAR2 Induction through Two Distinct Mechanisms
- Null Mutation in IRE1 Gene Inhibits Overproduction of Microsomal Cytochrome P450Alk1 (CYP 52A3) and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyces cerevisiae
- Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
- EFFCTS OF ALCOHOL AND PARTICLE CONCENTRATIONS ON LONGITUDINAL LIQUID DISPERSION COEFFICIENT IN GEL-PARTICLE-SUSPENDED BUBBLE COLUMN
- Perioperative Challenges Associated with a Pancreaticoduodenectomy and Distal Pancreatectomy for Pancreatic Cancer in Patients with Situs Inversus Totalis : Report of Two Cases
- Self-cloning of Genes for n-Alkane Assimilation from Candida maltosa(Microbiology & Fermentation Industry)
- Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
- Yeast Flocculation Mediated by Over-expression of the Chitin Synthase 2 Gene in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Purification of Cytochrome P-450alk from n-Alkane-grown Cells of Candida maltosa, and Cloning and Nucleotide Sequencing of the Encoding Gene(Microbiology & Fermentation Industry)
- Evidence That More Than One Gene Encodes n-Alkane-inducible Cytochrome P-450s in Candida maltosa, Found by Two-Step Gene Disruption(Microbiology & Fermentation Industry)
- Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in Saccharomyces cerevisiae
- Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
- A Protein Kinase C-Encoding Gene, pkcA, Is Essential to the Viability of the Filamentous Fungus Aspergillus nidulans
- Successful control of persistent hyperinsulinemic hypoglycemia of infancy with a high dextrin formula
- Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor
- Disruption of the SCS2 Ortholog in the Alkane-Assimilating Yeast Yarrowia lipolytica Impairs Its Growth on n-Decane, but Does Not Impair Inositol Prototrophy
- Involvement of LEM3/ROS3 in the Uptake of Phosphatidylcholine with Short Acyl Chains in Saccharomyces cerevisiae
- Myosin Motor-Like Domain of the Class VI Chitin Synthase CsmB Is Essential to Its Functions in Aspergillus nidulans
- The Dual Functions of Biphenyl-degrading Ability of Pseudomonas sp. KKS102 : Energy Acquisition and Substrate Detoxification(Food & Nutrition Science)
- Functional Differentiation of Chitin Synthases in Yarrowia lipolytica
- Treatments of Non-parasitic Giant Hepatic Cysts.
- Stabilization by the Mini-F Fragment of a pBR322 Derivative Bearing the Tryptophan Operon in Escherichia coli
- Subcloniag and Nucleotide Sequencing of an ARS Site of Candida maltosa Which Also Functions in Saccharomyces cerevisiae
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development.
- Isolation and Characterization of ECT1 Gene Encoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae.
- Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.
- Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase.