Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Saccharomyces cerevisiae cho1/pss mutants, which are severely impaired in phosphatidylserine (PS) synthesis, do not have detectable amounts of PS in their lipid fractions. Their derivatives with mutations that cause defects in tryptophan synthesis grew poorly in a medium containing 5μg/ml of L-tryptophan, a concentration that met the requirements of tryptophan-auxotrophic CHO1/PSS strains. The rates of tryptophan uptake of trp1 cho1/pss mutants were low at low tryptophan concentrations. This defect in the use of tryptophan was restored either by expression of CHO1/PSS or by introduction of a gene encoding tryptophan transporter, TAT1 or TAT2. These results indicate that PS synthesis is required for the maximal tryptophan-transporting activity of S. cerevisiae at low tryptophan concentrations.
- 社団法人日本農芸化学会の論文
- 2000-01-23
著者
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SHIBUYA Isao
Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo
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Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Nakamura H
Sci. Univ. Tokyo Tokyo Jpn
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NAKAMURA Hidemitsu
Department of Biotechnology, The University of Tokyo
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MIURA Keiji
Department of Molecular Biology, Saitama University
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FUKUDA Yu
Department of Molecular Biology, Saitama University
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Fukuda Yu
Department Of Molecular Biology Saitama University:(present Address)department Of Biochemistry Insti
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Miura Keiji
Department Of Molecular Biology Saitama University:(present Address)institute For Comprehensive Medi
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Shibuya Isao
Department Of Molecular Biology Saitama University
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Shibuya Isao
Department Of Dental Biomaterials Nihon University School Of Dentistry At Matsudo
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Ohta A
Dep. Of Biotechnology The Univ. Of Tokyo
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Ohta A
Univ. Tokyo Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakamura Hidemitsu
Department Of Biotechnology The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Nakamura H
Department Of Biotechnology The University Of Tokyo
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Ogura A
Meiji University
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Takahi Masamichi
Department Of Biotechnology The University Of Tokyo
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SHIBUYA Isao
Department of Biochemistry, Saitama University
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