Myosin Motor-Like Domain of the Class VI Chitin Synthase CsmB Is Essential to Its Functions in Aspergillus nidulans
スポンサーリンク
概要
- 論文の詳細を見る
Chitin is one of the major cell wall components of ascomycete filamentous fungi, and chitin synthesis plays important roles in the morphogenesis of hyphae. In the Aspergillus nidulans genome, there are two genes, csmA and csmB, that encode a myosin motor-like domain (MMD) at their N-termini and a chitin synthase domain (CSD) at their C-termini. In our previous studies, we found that the MMD of CsmA was required for its functionality, and that CsmA and CsmB had certain overlapping functions essential for polarized filamentous growth. In this study, we constructed a strain that produced CsmB lacking the MMD (CSΔMB). This strain exhibited defects similar to those of the csmB deletion mutant. FLAG-tagged CSΔMB (CSΔMB-FLAG) did not properly localize to the hyphae. CsmB was co-immunoprecipitated with actin in vivo, whereas CSΔMB was not. These results suggest that the MMD of CsmB is crucial for its proper localization via interaction with actin.
- 社団法人 日本農芸化学会の論文
- 2009-05-23
著者
-
Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
-
Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
-
Horiuchi Hiroyuki
Department Of Biotechnology The University Of Tokyo
-
TSUIZAKI Makusu
Department of Biotechnology, The University of Tokyo
-
TAKESHITA Norio
Department of Biotechnology, The University of Tokyo
-
Tsuizaki Makusu
Department Of Biotechnology The University Of Tokyo
-
Takeshita Norio
Department Of Biotechnology The University Of Tokyo
関連論文
- Evidence That the Expression of the Gene for NADPH-Cytochrome P-450 Reductase is n-Alkane-inducible in Candida maltosa
- Evolutionary Position of n-Alkane-assimilating Yeast Candida maltosa Shown by Nucleotide Sequence of Small-subunit Ribosomal RNA Gene
- Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
- Isolation and Characterization of ECT1 Gene Gncoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae^1
- R-Dependency of the Hardness Of Perovskite-Type RRh_3B Compounds (R = La, Gd, Lu and Sc) : Structure and Mechanical and Thermal Properties of Condensed Matter
- Solid Solution Range of Boron, Microhardness and Magnetic Properties of the Perovskite-Type GdRh_3B Obtained by Arc-melting Synthesis
- Nonparasitic solitary huge liver cysts causing intracystic hemorrhage or obstructive jaundice
- Xanthogranulomatous Cholecystitis with a Liver Abscess and Metastatic Endophthalmitis : Report of a Case
- Production of Recombinant Der fI (a major mite allergen) by Aspergillus oryzae
- Production of Recombinant Der fI with the Native IgE-Binding Activity Using a Baculovirus Expression System
- Construction of an Insertion Vector for Gene Targeting of Chicken Lens-specific Gene
- Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
- Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
- Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
- Expression of Plant Group 2 and Group 3 lea Genes in Saccharomyces cerevisiae Revealed Functional Divergence among LEA Proteins
- Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
- Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
- Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
- Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
- Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
- Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
- Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
- Transformation of a Leu^- Mutant of Rhizopus niveus with the leuA Gene of Mucor circinelloides
- Preparation of Protoplasts of Rhizopus niveus and Their Transformation with Plasmid DNA(Microbiology & Fermentation Industry)
- High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Isolation of a Genomic Clone Encoding RNase Rh of Rhizopus niveus and Characterization of Its 5-Terminal Region
- Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
- The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
- Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Misoflded Membrane-Bound Cytochrome P450 Activates KAR2 Induction through Two Distinct Mechanisms
- Null Mutation in IRE1 Gene Inhibits Overproduction of Microsomal Cytochrome P450Alk1 (CYP 52A3) and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyces cerevisiae
- EFFCTS OF ALCOHOL AND PARTICLE CONCENTRATIONS ON LONGITUDINAL LIQUID DISPERSION COEFFICIENT IN GEL-PARTICLE-SUSPENDED BUBBLE COLUMN
- Perioperative Challenges Associated with a Pancreaticoduodenectomy and Distal Pancreatectomy for Pancreatic Cancer in Patients with Situs Inversus Totalis : Report of Two Cases
- Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
- Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in Saccharomyces cerevisiae
- Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
- A Protein Kinase C-Encoding Gene, pkcA, Is Essential to the Viability of the Filamentous Fungus Aspergillus nidulans
- Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor
- Disruption of the SCS2 Ortholog in the Alkane-Assimilating Yeast Yarrowia lipolytica Impairs Its Growth on n-Decane, but Does Not Impair Inositol Prototrophy
- Phase Transition Study of C_ Crystal at around 340 K by Single-Crystal X-Ray Diffraction
- Involvement of LEM3/ROS3 in the Uptake of Phosphatidylcholine with Short Acyl Chains in Saccharomyces cerevisiae
- Myosin Motor-Like Domain of the Class VI Chitin Synthase CsmB Is Essential to Its Functions in Aspergillus nidulans
- The Dual Functions of Biphenyl-degrading Ability of Pseudomonas sp. KKS102 : Energy Acquisition and Substrate Detoxification(Food & Nutrition Science)
- Identification of Fibronectin in Chicken Thrombocytes
- Functional Differentiation of Chitin Synthases in Yarrowia lipolytica
- A Case of Cancer of the Ampulla of Vater Accompanied by Malrotation
- Treatments of Non-parasitic Giant Hepatic Cysts.
- Stabilization by the Mini-F Fragment of a pBR322 Derivative Bearing the Tryptophan Operon in Escherichia coli
- Schwannoma in the Sigmoid Colon. Report of a Case.
- Chicken Monoclonal Antibodies against Synthetic Bovine Prion Protein Peptide.
- Adult Colonic Intussusception: A Case Report.
- Development of intrahepatic cholangiocarcinoma after a 14-year follow-up of a patient with primary sclerosing cholangitis and ulcerative colitis
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development.
- Isolation and Characterization of ECT1 Gene Encoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae.
- Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.
- Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase.