High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
The gene encoding an extracellular Rhizopus niveus aspartic proteinase I (RNAP-I) was introduced into Saccharomyces cerevisiae. The yeast cell carrying a plasmid containing the intact RNAP-I gene under the control of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene promoter of S. cerevisiae did not synthesize RNAP-I at all. On the other hand, when the intron of the RNAP-I gene had been removed from the gene in the plasmid, the cell secreted RNAP-I with high efficiency. Processing of the pro-sequence occurred at the same region of the pro-enzyme during cultivation as observed in the culture of R. niveus. Moreover, the promoter and the terminator of the original RNAP-I gene were found to be weakly functional in the yeast cell with respect to expression of the intronless RNAP-I gene, although the initiation and termination sites were heterogeneous. The effects of vector-types on the extracellular production of RNAP-I were also investigated.
- 社団法人日本農芸化学会の論文
- 1990-07-23
著者
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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ASHIKARI Toshihiko
Institute for Advanced Technology, Suntory Ltd.
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YANO KEIJI
Department of BioEngineering, Nagaoka University of Technology
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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AMACHI Teruo
Institute for Fundamental Research, Research Center
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YOSHIZUMI Hajime
Institute for Fundamental Research, Research Center
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Yoshizumi Hajime
Department Of Food Science And Nutrition Faculty Of Agriculture Kinki University
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Yoshizumi Hajime
Institute For Fundamental Research Research Center
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Yoshizumi Hajime
Kinki University
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Yanai Koji
Department Of Biotechnology The University Of Tokyo:bio Science Laboratories Meiji Seika Kaisha Ltd.
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Amachi T
Kyoto Univ. Kyoto Jpn
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Amachi Teruo
Institute For Fundamental Research Research Center Suntory Ltd.
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Ashikari Toshihiko
Institute For Advanced Technology Suntory Ltd.
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