Evaluation of the Function of Arming Yeast Displyaing Glucoamylase on Its Cell Surface by Direct Fermentation of Corn to Ethanol

概要

論文の詳細を見る
We have constructed a Saccharomyces cerevisiae strain displaying glucoamylase from Rhizopus oryzae on its cell surface, which could grow on soluble starch as the sole source of carbon and energy(Murai et al., Appl.Environ.Microbiol., 63, 1362-1366, 1977). This system was introduced into a yeast strain, G-1315, with a high fermentation ability, and the direct production of ethanol from corn was examined. When a transformant of strain G-1315 displaying glucoamylase on the cell surface was cultivated statically at 27℃ for 7 d using ground raw corn as starchy material, ethanol was produced at a concentration of 2.34%(w/w). Addition of an α-amylase reagent(Termamil)to liquefy the ground corn prior to fermentation improved the ethanol production to 5.32%(w/w), which was comparable to that of transformants secreting glucoamylase. These results demonstrate that the "arming yeast" developed by a cell surface engineering technique and its further improvement is applicable to direct fermentation of starchy materials.
社団法人日本生物工学会の論文
1998-12-25