Studies on the Utilization of Hydrocarbons by Microorganisms : (II) Production of Cytochrome c by Candida albicans in Hydrocarbon Fermentation : (Part II) Production of Cytochrome c by Candida albicans
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概要
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This paper dials with the study on the relations among the growth rate, cytochrome c content and respiratory activity of Candida albicans when the yeast was cultivated on n-hexadecane under aeration.Under the cultural conditions employed, the maximal cytochrome c content was about 1.5mg per g dry cells, which was analogous to that of glucosegrown cells.The growth rate was closely related with the cytochrome c content. The latter increased in pallalel with the yeast growth, reached to the maximal value at the later part of the oxponential phase, then falled off cradually. The correlationship between the cytochrome c content and the specific growth rate showed that the former can be used as an index of the growth activity of the yeast.It has been observed that oxidative degradation of hydrocarbon by microorganisms is carried out under more aerobic conditions than that of carbohydrate. Hence our attention was called to the question whether the yeasts grown on hydrocarbons as a sole carbone source would produce a large amount of respiratory enzymesm especially cytochrome c. In this study we investigated the ability of hydrocarbon utilization and cytochrome c productivity of several kinds of yeasts, and found out that C. albicans was suitable for both purposes.Oxygen-uptake of C. almicans was inhibited by addition of sodium azide, and this suggested that the respiration of this yeast would proceed via an esectron transport system including at least cytochromes α_1 and α_3. Also, we could detect cytochromes a, b, and c in reduced intact cells spectrophotometrically by the opal-glass method of Shibata.We also attempted to study on the relation among grouth rate, cytochtome content, and respiratory activity, and to use the cytochrome c content as an index of growth activity of cells. The results obtained showed that the growth activity of the yeast correlated apparently with the cytochrome c content.Under the cultural conditions determined in our previous paper, we obtained a maximal cytochrome c content of 1.5mg. per g. dry cells of C. albicans.
- 公益社団法人日本生物工学会の論文
- 1967-12-25
著者
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Tanaka Atsuo
Department Of Applied Physics Faculty Of Engineering Osaka University
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Fukui Saburo
Department Of Applied Chemistry School Of Engineering Tokai University
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Maki Hidehiko
Department of Industrial Chemistry, Faculty of Engineering, Kyoto University
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Maki Hidehiko
Department Of Industrial Chemistry Faculty Of Engineering Kyoto University
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Fukui Saburo
Department of Industrial Chemistry, Faculty of Engineering, Kyoto University
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