Molecular and Biochemical Characterization of a Novel Hydroxycinnamoyl-CoA : Anthocyanin 3-O-Glucoside-6"-O-Acyltransferase from Perilla frutescens
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概要
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We have isolated and characterized a cDNA, PfAT208, encoding hydroxycinnamoyl-CoA: anthocyanin 3-O-glucoside-6"-O-acyltransferase(3AT) from Perilla frutescens. The identity of the cDNA was established by determination of the reaction products with recombinant enzyme overexpressed in Escherichia coli. The deduced amino acid sequence has a few regions that are conserved in a CoA-dependent acyltransferase family. The recombinant enzyme produced in yeast could utilize cyanidin 3-glucoside and cyanidin 3, 5-diglucoside, putative substrates in vivo, as well as other anthocyanins. The inhibitory effects of diethyl pyrocarbonate and N-ethylmaleimide on the recombinant 3AT activities suggest that histidine and cysteine residues are important for their catalytic function. These properties are in common with anthocyanin 5-O-glucoside-6"-O-acyltransferase(5AT). In Northern analysis, a transcript of PfAT208 was detected in the young leaves of perilla red forma. The properties of other cDNAs, gentian GAT106 and petunia PhAT48, isolated during the above cloning procedure are also described.
- 日本植物生理学会の論文
著者
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Ara Takeshi
Kazusa DNA Research Institute
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Tanaka Y
Suntory Ltd. Osaka Jpn
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ASHIKARI Toshihiko
Institute for Advanced Technology, Suntory Ltd.
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Tanaka Y
National Inst. Agrobiological Resources Tsukuba Jpn
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Ashikari T
Nihon Univ. Kanagawa Jpn
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TANAKA Yoshikazu
Institute for Geothermal Sciences Graduate School of Science, Kyoto University
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FUKUI Yuko
Institute for Advanced Technology, Suntory, Ltd.
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KUSUMI Takaaki
Institute for Advanced Technology, Suntory, Ltd.
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Yonekura-Sakakibara Keiko
Institute for Fundamental Research, Suntory Ltd.
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Fukuchi-Mizutani Masako
Institute for Fundamental Research, Suntory Ltd.
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Fujiwara Hiroyuki
Institute for Fundamental Research, Suntory Ltd.
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Murakami Yasuyuki
Institute for Fundamental Research, Suntory Ltd.
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Yamaguchi Masaatsu
Department of Food Science and Technology, Minami-Kyushu University
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Aoki T
Nihon Univ. Kanagawa Jpn
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Fujiwara Hiroyuki
Institute For Fundamental Research Suntory Ltd.
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Kusumi Takaaki
Suntory Limited Research Center Institute For Plant Science
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Fukui Yuko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Tanaka Yoshikazu
Central Res. Laboratories Hokko Chemical Ind. Co.
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Ashikari Toshihiko
Institute For Advanced Technology Suntory Ltd.
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Yonekura K
Kyushu Univ. Fukuoka Jpn
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Yamaguchi Masaatsu
Department Of Food Science And Technology Minami-kyushu University
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Yonekura-sakakibara Keiko
Institute For Fundamental Research Suntory Ltd
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Murakami Yasuyuki
Institute For Fundamental Research Suntory Ltd.
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Tanaka Yoshikazu
Central Research Laboratories Hokko Chemical Industry Co.
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Fukuchi-mizutani Masako
Institute For Plant Science Suntory Holdings Ltd.
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Tanaka Yoshikazu
Institute For Advanced Core Technology Suntory Limited
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Tanaka Yoshikazu
Institute For Advanced Core Technology
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Yonekura-Sakakibara Keiko
Institute for Advanced Research, Suntory, Ltd.
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Fukuchi-Mizutani Masako
Institute for Advanced Research, Suntory, Ltd.
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Fukui Yuko
Institute for Advanced Research, Suntory, Ltd.
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Kusumi Takaaki
Institute for Fundamental Research, Suntory Ltd.
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Tanaka Yoshikazu
Institute for Fundamental Research, Suntory Ltd.
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