Molecular characterization of the flavonoid biosynthetic pathway and flower color modification of Nierembergia sp
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概要
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Nierembergia sp., a popular floricultural species, only has violet and white flower color and lacks pink to red. To elucidate the reason, we analyzed its flavonoids. Its major anthocyanin was determined to be delphinidin 3-O-(6-O-(4-O-(4-O-(6-O-caffeoyl-β-D-glucopyranosyl)-p-coumaroyl)-α-L-rhamnosyl)-β-D-glucopyranosyl)-5-O-β-D-glucopyranoside. The petals rarely contained cyanidin and pelargonidin, and they contained more flavonols than anthocyanins. We also characterized the biosynthetic pathway by cloning the cDNAs encoding enzymes involved in the flavonoid biosynthesis pathway: chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), flavonoid 3′,5′-hydroxylase (F3′5′H), dihydroflavonol 4-reductase (DFR), flavonol synthase (FLS), and UDP-rhamnose: anthocyanidin 3-glucoside rhamnosyltransferase (3RT). Northern blot analysis revealed that the expressions of CHS, F3′5′H, DFR, and 3RT genes were coordinately regulated in parallel with anthocyanin accumulation in the petals, indicating that anthocyanin biosynthesis is transcriptionally regulated; on the other hand, the transcripts of the F3′H gene were rarely detected. Antisense suppression of the F3′5′H gene decreased the amount of F3′5′H transcripts and that of delphinidin. It was noteworthy that the color of the transgenic flower changed from violet to white rather than to reddish, which was the expectation.
- 日本植物細胞分子生物学会の論文
- 2006-03-01
著者
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IWASHITA TAKASHI
Suntory Institute for Bioorganic Research(SUNBOR)
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Tanaka Y
Suntory Ltd. Osaka Jpn
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Tanaka Y
National Inst. Agrobiological Resources Tsukuba Jpn
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TANAKA Yoshikazu
Institute for Geothermal Sciences Graduate School of Science, Kyoto University
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FUKUCHI MIZUTANI
Institute for Advanced Technology, Suntory, Ltd.
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UEYAMA Yukiko
Institute for Advanced Technology, Suntory, Ltd.
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FUKUI Yuko
Institute for Advanced Technology, Suntory, Ltd.
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KUSUMI Takaaki
Institute for Advanced Technology, Suntory, Ltd.
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KATSUMOTO Yukihisa
Institute for Advanced Research, Suntory, Ltd.
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UEYAMA Yukiko
Division of Molecular Science, Graduate School of Science and Technology, Kobe University
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OHKAWA Hideo
Institute for Advanced Technology, Suntory, Ltd.
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Ohkawa Hideo
Biotechnology Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.
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Ohkawa Hideo
Institute For Advanced Technology Suntory Ltd.
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Iwashita Takashi
Suntory Inst. For Bioorganic Res. Osaka Jpn
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Kusumi Takaaki
Suntory Limited Research Center Institute For Plant Science
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Kusumi Takaaki
R&d Department Suntory Flowers Ltd.
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Fukui Yuko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Ueyama Yukiko
Institute For Advanced Technology Suntory Ltd.
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Ohkawa H
Institute For Advanced Technology Suntory Ltd.
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Tanaka Yoshikazu
Central Res. Laboratories Hokko Chemical Ind. Co.
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Katsumoto Yukihisa
Inst. For Plant Sci. Suntory Holdings Ltd.
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Kusumi T
Institute For Fundamental Research Suntory Ltd
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Tanaka Yoshikazu
Central Research Laboratories Hokko Chemical Industry Co.
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Okuhara Hiroaki
Institute For Plant Science Suntory Holdings Ltd.
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Fukuchi-mizutani Masako
Institute For Plant Science Suntory Holdings Ltd.
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Tanaka Yoshikazu
Institute For Advanced Core Technology Suntory Limited
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Tanaka Yoshikazu
Institute For Advanced Core Technology
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Fukui Yuko
Institute for Advanced Research, Suntory, Ltd.
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