Molecular Cloning and Characterization of Rosa hybrida Dihydroflavonol 4-reductase Gene : GENE STRUCTURE AND EXPRESSION

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概要

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• A full length cDNA clone encoding rose dihydroflavonol 4-reductase (DFR) was isolated from a cDNA library derived from rose petals by screening with the cDNA of Petunia hybrida DFR. Sequence comparison of the rose DFR with reported DFR genes revealed that they are homologous to each other. The amount of DFR mRNA in rose petals was developmentally regulated and paralleled anthocyanin production in petals. Sepals, thorns, styles and stamens also contained anthocyanins and DFR mRNA. No DFR mRNA was observed in mature leaves and a small amount of the transcript was detected in young leaves. A petunia cultivar, whose colour was pale pink due to a deficiency in flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase, was transformed with a binary vector containing a rose DFR cDNA cloned behind a constitutive promoter. Petals and anthers of the resultant transgenic petunia plants were salmon pink and contained pelargonidin, an anthocyanidin rarely found in petunia.

• 日本植物生理学会の論文

著者

• Tanaka Y

  Suntory Ltd. Osaka Jpn

• Tanaka Y

  National Inst. Agrobiological Resources Tsukuba Jpn

• TANAKA Yoshikazu

  Institute for Geothermal Sciences Graduate School of Science, Kyoto University

• FUKUI Yuko

  Institute for Advanced Technology, Suntory, Ltd.

• KUSUMI Takaaki

  Institute for Advanced Technology, Suntory, Ltd.

• HOLTON Timothy

  Florigene, Ltd.

• Fukuchi-Mizutani Masako

  Institute for Fundamental Research, Suntory Ltd.

• Higgins Elizabeth

  Florigene Pty Ltd.

• Kusumi Takaaki

  Suntory Limited Research Center Institute For Plant Science

• Kusumi Takaaki

  R&d Department Suntory Flowers Ltd.

• Holton Timothy

  Florigene Pty. Ltd

• Fukui Yuko

  Inst. For Plant Sci. Suntory Holdings Ltd.

• Tanaka Yoshikazu

  Central Res. Laboratories Hokko Chemical Ind. Co.

• Kusumi T

  Institute For Fundamental Research Suntory Ltd

• Tanaka Yoshikazu

  Central Research Laboratories Hokko Chemical Industry Co.

• Fukuchi-mizutani Masako

  Institute For Plant Science Suntory Holdings Ltd.

• Tanaka Yoshikazu

  Institute For Advanced Core Technology Suntory Limited

• Tanaka Yoshikazu

  Institute For Advanced Core Technology

• Fukuchi-Mizutani Masako

  Institute for Advanced Research, Suntory, Ltd.

• Fukui Yuko

  Institute for Advanced Research, Suntory, Ltd.

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