Flower color modification of Petunia hybrida commercial varieties by metabolic engineering
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概要
- 論文の詳細を見る
Petunia flower colors are mainly due to flavonoids. The flower color of commercial varieties of Petunia hybrida was successfully modified by the suppression of endogenous flavonoid biosynthetic genes, the expression of a hetelorogous flavonoid biosynthetic gene, and the combination of both. Flower color changed from purple to almost white or from purple to red by the suppression of the endogenous gene expression, from red to orange by the down-regulation of the flavonoid 3′-hydroxylase gene and the expression of the rose dihydroflavonol 4-reductase gene, and from violet to pale violet by the expression of the flavonol synthase or flavone synthase gene. These results clearly indicate the usefulness of metabolic engineering of the flavonoid biosynthetic pathway to modify flower color. Only a few of the transgenic petunia exhibited phenotypic stability. For commercialisation, it is necessary to generate many independent transgenic lines, select elite lines with stable phenotypes and maintain them in tissue culture.
- 日本植物細胞分子生物学会の論文
- 2004-12-01
著者
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Tanaka Y
Suntory Ltd. Osaka Jpn
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Tanaka Y
National Inst. Agrobiological Resources Tsukuba Jpn
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TANAKA Yoshikazu
Institute for Geothermal Sciences Graduate School of Science, Kyoto University
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NAKAMURA Noriko
Institute for Advanced Technology, Suntory, Ltd.
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FUKUCHI MIZUTANI
Institute for Advanced Technology, Suntory, Ltd.
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UEYAMA Yukiko
Institute for Advanced Technology, Suntory, Ltd.
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YONEKURA SAKAKIBARA
Institute for Advanced Technology, Suntory, Ltd.
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FUKUI Yuko
Institute for Advanced Technology, Suntory, Ltd.
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TSUDA Shinzo
Institute for Advanced Research, Suntory, Ltd.
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KATSUMOTO Yukihisa
Institute for Advanced Research, Suntory, Ltd.
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OHIRA Kazuyuki
Institute for Advanced Research, Suntory, Ltd.
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UEYAMA Yukiko
Kobe University
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OHKAWA Hideo
Kobe University
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HOLTON Timothy
Florigene, Ltd.
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KUSUMI Takaaki
Suntory Flowers, Ltd.
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OKAZAKI Koei
Kazusa DNA Research Institute
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Nakamura Noriko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Ohkawa Hideo
Biotechnology Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.
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Ohkawa Hideo
Institute For Advanced Technology Suntory Ltd.
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Okazaki Koei
Kazusa Dna Res. Inst.
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Yonekura Sakakibara
Suntory Limited Research Center Institute For Plant Science
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Kusumi Takaaki
Suntory Limited Research Center Institute For Plant Science
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Kusumi Takaaki
R&d Department Suntory Flowers Ltd.
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Holton Timothy
Florigene Pty. Ltd
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Fukui Yuko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Tsuda Shinzo
Inst. For Plant Sci. Suntory Holdings Ltd.
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Ueyama Yukiko
Institute For Advanced Technology Suntory Ltd.
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Ohkawa H
Institute For Advanced Technology Suntory Ltd.
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Tanaka Yoshikazu
Central Res. Laboratories Hokko Chemical Ind. Co.
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Katsumoto Yukihisa
Inst. For Plant Sci. Suntory Holdings Ltd.
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Kusumi T
Institute For Fundamental Research Suntory Ltd
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Tanaka Yoshikazu
Central Research Laboratories Hokko Chemical Industry Co.
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Okuhara Hiroaki
Institute For Plant Science Suntory Holdings Ltd.
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Fukuchi-mizutani Masako
Institute For Plant Science Suntory Holdings Ltd.
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Tanaka Yoshikazu
Institute For Advanced Core Technology Suntory Limited
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Tanaka Yoshikazu
Institute For Advanced Core Technology
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Nakamura Noriko
Institute for Advanced Research, Suntory, Ltd.
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Fukui Yuko
Institute for Advanced Research, Suntory, Ltd.
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