Biochemical and molecular characterization of anthocyanidin/flavonol 3-glucosylation pathways in Rosa×hybrida
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概要
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Rose petals contain 3-glucosylated anthocyanidins and flavonols. We isolated three flavonoid 3-glucosyltransferase (UF3GT) homolog genes (RhUF3GT1, RhUF3GT2, and RhUF3GT3) from rose. RhUF3GT1 encoded an amino acid sequence that is almost identical to the reported rose partial UF3GT homologs and highly homologous to strawberry and apple UF3GTs. Recombinant RhUF3GT1 expressed in yeast catalyzes 3-glucosylation of anthocyanidins but not flavonols. RhUF3GT1 was not expressed in the petals of many cultivars even when anthocyanin biosynthesis was active, while it was expressed in the mature petals of cultivars that synthesize cyanidin 3-glucoside in the mature petals. RhUF3GT2 and RhUF3GT3, sharing 79% identity, exhibit only 42% and 41% identities to RhUF3GT1, respectively, and are distantly related to strawberry and apple UF3GTs. They were expressed in coordination with the flavonol synthase gene in the petal. The recombinant RhUF3GT2 expressed in yeast catalyzed 3-glucosylation of flavonol much more efficiently than that of anthocyanidins. We suggest that RhUF3GT2 catalyzes flavonol 3-glucosylation in rose petals and that it also contributes to accumulation of anthocyanidin 3-glucoside in the petals.
- 2011-03-25
著者
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Tanaka Y
Suntory Ltd. Osaka Jpn
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Tanaka Y
National Inst. Agrobiological Resources Tsukuba Jpn
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TANAKA Yoshikazu
Institute for Geothermal Sciences Graduate School of Science, Kyoto University
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NAKAMURA Noriko
Institute for Advanced Technology, Suntory, Ltd.
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FUKUCHI MIZUTANI
Institute for Advanced Technology, Suntory, Ltd.
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TOGAMI Junichi
Institute for Advanced Technology, Suntory, Ltd.
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ISHIGURO Kanako
Institute for Advanced Technology, Suntory, Ltd.
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FUKUI Yuko
Institute for Advanced Technology, Suntory, Ltd.
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KATSUMOTO Yukihisa
Institute for Advanced Research, Suntory, Ltd.
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Fukuchi-Mizutani Masako
Institute for Fundamental Research, Suntory Ltd.
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Nakamura Noriko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Fukui Yuko
Inst. For Plant Sci. Suntory Holdings Ltd.
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Tanaka Yoshikazu
Central Res. Laboratories Hokko Chemical Ind. Co.
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Katsumoto Yukihisa
Inst. For Plant Sci. Suntory Holdings Ltd.
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Akagi Misako
Institute for Plant Science, Suntory Holdings Ltd.
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Aida Mitsuhiro
Institute For Plant Science Suntory Holdings Ltd.
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Tanaka Yoshikazu
Central Research Laboratories Hokko Chemical Industry Co.
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Fukuchi-mizutani Masako
Institute For Plant Science Suntory Holdings Ltd.
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Ishiguro Kanako
Institute For Plant Science Suntory Holdings Ltd.
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Togami Junichi
Institute For Plant Science Suntory Holdings Ltd.
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Tanaka Yoshikazu
Institute For Advanced Core Technology Suntory Limited
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Tanaka Yoshikazu
Institute For Advanced Core Technology
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Fukuchi-Mizutani Masako
Institute for Advanced Research, Suntory, Ltd.
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Nakamura Noriko
Institute for Advanced Research, Suntory, Ltd.
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Fukui Yuko
Institute for Advanced Research, Suntory, Ltd.
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